You will require a plate reader capable of reading FAM, HEX, ATTO 550, ATTO 647N and ROX (the wavelengths of which can be found in our PACE Multiplex Master Mix User Guide). It is important that filters used in the plate reader have narrow bandwidths (10 nm) to avoid cross fluorescence. Alternatively, plate readers using monochromators capable of using at least five channels with 10 nm bandwidth will also work.
