Our patented genotyping chemistry, PACE, combines competitive allele-specific PCR with multiple advances on existing genotyping marker technologies such as KASP™ and Amplifluor®. PACE Genotyping Master Mixes are designed for use with PACE assays and can also be used directly with existing KASP and Amplifluor assays.
Unlike probe-based products such as TaqMan™, PACE assays only require un-labelled primer oligos to make up the PACE genotyping reaction, in combination with PACE Genotyping Master Mix. Watch our quick video explaining how PACE genotyping works.
All our master mixes are supplied at 2x concentration for convenience and contain ROX normalising dye at either high (500 nM working concentration), low (25 nM working concentration), standard ROX level (150 nM working concentration) or without ROX.
3CR Bioscience provides a free PACE assay design service plus full- or partial assay validation options to help you reduce the cost and turnaround time of your genotyping.
ProbeSure™ Genotyping Master Mix is designed for use with 5’ nuclease assays and is suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes. ProbeSure Genotyping Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.
ProbeSure Genotyping Master Mix has been carefully optimised to work over a wide dynamic range of reaction volumes and sample template qualities. ProbeSure Genotyping Master Mix combines class-leading performance with the most competitive prices in the market.