PACE OneStep RT-PCR Master Mix is designed for highly specific and sensitive one-step reverse transcriptase (RT) fluorescently-reporting endpoint PCR using RNA template.
PACE OneStep RT-PCR Master Mix includes optimised components which allow reverse transcription and subsequent PCR amplification to take place in the same reaction well.
The PACE OneStep RT-PCR reaction is comprised of three parts:
- Assay Mix (unlabelled, forward, and reverse primers designed to amplify the target region)
- Reverse transcriptase enzyme
- PACE OneStep RT-PCR master Mix containing all components required for PCR amplification (post reverse-transcription) and the generation of fluorescent signals.
When combined with template RNA, these components create a PACE OneStep RT-PCR.