Genotyping Technology in Action

See in more detail how our genotyping technology, products and services are most commonly applied in the field by scientists just like you, and the competitive advantages they provide.

genotyping technolOGY APPLICATIONS

3CR Bioscience is dedicated to developing cutting-edge innovation and exceptional products for the life sciences, with an emphasis on PCR genotyping technology and molecular markers for plant, animal, human and microbial genetic research.

From the developers of KASP™ and PACE® technology, PACE Genotyping Master Mix continues our pedigree of innovation and exceptional performance, characterised by extremely low non-specific amplification, and universal compatibility with allele-specific PCR assays including PACE, KASP, and Amplifluor®. Full functionality at all reaction volumes and plate-well densities (96-, 384-, 1536-well and array tape), our reagents undergo rigorous quality control and tracking procedures. All batches of mix are performance tested and certified, with Certificate of Analysis available for every batch, available to download from our website.

ProbeSure™ Genotyping Master Mix combines class-leading performance with the most competitive prices in the market. Our ProbeSure Genotyping Master Mix is designed for use with 5’ nuclease assays and is suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes. ProbeSure Genotyping Master Mix has been carefully optimised to work over a wide dynamic range of reaction volumes and sample template qualities. 

In an era of rapid breakthroughs in genomics, our hub of scientific excellence with 100+ years of combined experience in developing high-performance molecular biology tools is perfectly positioned to help you advance rapidly towards your goals.

We have successfully converted thousands of validated SNP targets into breeder-friendly markers for marker-assisted-selection studies across the globe. Let us help you avoid common pitfalls of marker assay design with our free allele-specific PACE assay design service and full-or partial assay validation options. All that is needed is short sequence information surrounding the variant of interest (e.g., SNP or Indel) and a qPCR machine or fluorescent plate reader.

Ready to run? For PACE®, KASP™ and any other similar PCR markers, our patented PACE Genotyping Master Mix gives exceptional performance, characterised by its extremely low non-specific amplification, and universal compatibility with allele-specific PCR assays including PACE, KASP, and Amplifluor®. 3CR Bioscience’s PACE Genotyping Master Mix provides unparalleled accuracy, affordability and reliability at any reaction volume, making it scalable and simple to integrate efficiently into any sized study, small or large.

DNA purification steps can be costly and time-consuming, especially when processing thousands of samples within tight deadlines, as many of our customers do. With the latest PACE® 2.0 formulation, you can now obtain superior data from your samples without the need for extensive purification.

PACE 2.0 Genotyping Master Mix ensures an unrivalled signal-to-noise ratio and produces tight clusters even when working with high-throughput, crude DNA preps, resulting in consistently exceptional performance. Efficiently streamline your workflow and reduce costs without compromising the quality of your results.

PACE 2.0 Genotyping Master Mix excels in handling crude DNA samples, making it the ideal solution for challenging starting material. PACE 2.0 has been specially formulated to overcome the obstacles presented by common PCR inhibitor compounds, such as phenols and tannins found in plant material. Even notoriously tricky samples, like oil palm and conifers, can be reliably assayed using hot shot or other crude DNA prep methods and still deliver reliable and accurate data.

3CR Bioscience has developed precision solutions for rapid pathogen detection, applicable right across agricultural, veterinary, and medical disciplines.

We have taken our patented PACE® chemistry one step further and created PACE OneStep RT-PCR, a fluorescent PCR detection system that can be customised to detect any DNA or RNA-based pathogens. Recent examples include the rapid, large-scale detection of agricultural disease outbreaks and the monitoring of circulating SARS-COV-2 variants within a population.

PACE OneStep RT-PCR has been adopted as a novel, rapid screen for the single-stranded RNA Cotton leafroll dwarf virus (CLRDV) in cultivated cotton by a group at Texas A&M University (read publication). CLRDV is a single-stranded RNA virus that poses a major global threat to cotton production, and current detection methods are expensive and time-consuming. PACE OneStep RT-PCR Master Mix (RT relating to the addition of reverse transcriptase) combines RNA transcription and cDNA amplification into a single reaction, using sequence-specific fluorescent primers to differentiate between infected and uninfected samples. PACE OneStep RT-PCR Master Mix can be applied to screens for DNA or RNA viruses in other crops and even animal species by targeting conserved virus- and host-specific genes.

Beyond pathogen detection, PACE OneStep RT-PCR genotyping has been adapted as a highly sensitive and accurate tool to rapidly identify variants within a circulating SARS-COV-2 population.

During the recent SARS-CoV-2 outbreaks, tracking genetic variations from positive samples has yielded crucial information about the number of variants circulating in each new outbreak and the possible lines of transmission. However, conventional screening by sequencing high numbers of positive SARS-CoV-2 samples is costly for population-scale test and trace operations.

A PACE OneStep RT-PCR Master Mix SNP identification pipeline, developed by School of Biological Sciences at the University of Bristol with 3CR Bioscience, offers a high-throughput, accurate, and affordable means to screen positive SARS-CoV-2 samples and monitor SNP variants in an outbreak. The publicly available pipeline is periodically updated as viral genotypes arise or disappear from circulation. The flexibility and versatility of PACE OneStep RT-PCR Master Mix means existing SNP panels can be quickly and inexpensively modified to keep pace with changes in strain emergence, providing valuable insight for epidemiological studies.

PACE® Multiplex Master Mix is a powerful and versatile extension of our PACE 2.0 Genotyping Master Mix, allowing for the simultaneous detection of multiple targets within a single PCR reaction.

Genotyping with PACE Multiplex Master Mix combines the principles of PCR amplification with the specificity of allele-specific extension and fluorescent labelling. This breakthrough evolution of our flagship technology enables researchers to assess multiple genotypes in a single reaction, reducing time, cost, and the need for additional samples and consumables. PACE Multiplex Master Mix genotyping has widespread applications in fields such as marker-assisted selection, transgenic plant development, seed testing, and disease diagnostics. Recent customer applications include quad-allelic SNP detection in cancer diagnostics, adventitious presence sequence detection in seed quality control testing, and pathogen detection on commercially grown crops. The flexibility and scalability of PACE Multiplex Master Mix make it an indispensable genotyping tool for higher throughput requirements.

At 3CR Bioscience, we are dedicated to your success and committed to supporting you throughout your genotyping journey. Our team of experts is always available to assist you at any stage, from assay design and set up to running the experiments and data analysis.

Assay design: Our team has been designing PCR genotyping technology assays for decades, and we are happy to share our expertise with you to achieve assays with optimal performance and accuracy. We offer a free PACE® assay design service for customers to save time and help reduce the cost and turnaround time of your genotyping. From assay validation to protocol implementation, we provide comprehensive technical support, addressing any challenges that arise. There isn’t much we can’t solve, and our dedication and customer communication are second to none.

Technical resources: Count on us for comprehensive resources and guidance during assay running and data analysis. Troubleshooting is part and parcel of what we do in our own labs and for our customers – over the years, we have seen it all! Our aim is to empower you with the skills and knowledge that will maximise the rapid production of top-quality data routinely in your facility, regardless of its size or your level of experience.

Our results in numbers

Just some of the reasons why we are technology leaders in PCR genotyping technology and Making Science Affordable

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Industries

Industries using our technology

Plant Genomics

Paving the way for protected plant diversity, sustainable agriculture, and enhanced crop traits with PACE Genotyping

PACE genotyping is an invaluable tool for plant breeders and researchers. Develop SNP and Indel markers easily for rapid, precision characterisation of desirable traits in plants, such as improved yield, resistance to pests, diseases, abiotic stress and enhanced nutritional qualities.

With 3CR Bioscience you can develop breeder-friendly markers for accelerated genetic crossing and crop improvement in marker-assisted breeding programs. Applications also include genetic mapping, sex determination and marker-assisted selection to accelerate the development of improved plant varieties. PCR genotyping is also a powerful tool for plant biology and conservation research investigating genetic diversity, relatedness, and population structure.

Genotyping technology - wheat crop breeding genomics

ANIMAL breeding

Advancing animal health, breeding and conservation with PACE genotyping

PACE genotyping is widely used to identify and select animals with desirable traits. Accurate tracking of SNPs and Indels associated with traits such as disease resistance, productivity, or reproductive performance allows breeders to make informed decisions for breeding pair selection. Accelerate the development of improved animal breeds and enhance your breeding programs with the help our technology.

PACE genotyping can also be used to genotyping large numbers of animals quickly and accurately with identified allelic variants to study genetic relationships, evolutionary history, and patterns of genetic variation within and across animal populations. Generate rapid, precision data for conservation efforts, to understand species evolution and design breeding strategies. In addition, efficient genotyping workflows can be integrated for veterinary genetic studies and diagnostics to help identify genetic disorders and determine the risk to animals.

Sheep Breeding Program. Sheep at farm.

HUMAN GENOMICS

Genotyping at the frontiers of human health, investigating human genomics and disease

With our rapid, accurate technology for analysing gene variants such as SNPs and Indels, researchers can investigate genetic predispositions to diseases such as cancer, cardiovascular disorders, neurological conditions and more. We assist researchers discovering novel disease- and disorder-associated genetic markers. The analysis of genetic variants can play a crucial role in population and epidemiology studies, identifying and tracking variations that contribute to individual differences in susceptibility, treatment response and diseases outcomes.

Our genotyping technology is applicable to microbial identification and outbreak investigation, spreading light on the spread and evolution of pathogens such as SARS-CoV-2, informing surveillance and control measures.

Genotyping from crude DNA samples
Great assistance, another role model of fast service. I was impressed with such high work efficiency."
Crop Researcher,
Global Commercial Grower
The PACE assay designed for Barley yellow dwarf virus (BYDV) worked out great, we now have a diagnostic marker for BYDV which is so much better than the SSR we currently use! We have tested it with both wheat and wheat grass lines in our breeding program and got perfect results."
Research Scientist,
University of Minnesota
Thank you for all your technical assistance and excellent customer service."
Research Scientist,
University of Minnesota
Cannabinoid type is largely governed by a single locus, and with PACE assays, we can screen thousands of plants for chemotype in a timeline of days rather than months using HPLC, and at a fraction of the cost."
Research Scientist,
Cornell University
I am very impressed with 3CR Bioscience and their PACE Genotyping Master Mix which we tested on several plant species. Their shipment is very speedy. The assay design service from the Technical Support team was very efficient in responding to my request. Thank you!"
Laboratory Service Lead,
Commercial Seed Producer
Thanks for your excellent customer service and quick turnaround times"
Researcher,
University of California, Davis
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Legal Information
TaqMan™ is a registered trademark of Roche Molecular Systems, Inc.
Zen™ Probe is a registered trademark of Integrated DNA Technologies, Inc.
KASP™, BHQ™ Probes, and BHQplus™ Probes are trademarks of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA

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Contact us

MEET OUR TEAM

Steve Asquith Managing Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John Holme Technical Director
John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base. During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Dr. Nisha JainOperations Director
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP. Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nazma Saffin General Manager
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Jon Curtis Non-Executive Chair
After 8 years in The Royal Air Force, Jon moved to the Imperial Cancer Research Fund where he pioneered the use of ultra high-throughput genomic automation, capable of 46,000 PCRs per hour. In the 1990’s Jon joined GlaxoSmithKline, implementing a high-throughput genomics platform into their drug discovery pipeline. Whilst there he also developed acoustic mixing into compound management, becoming the gold standard across pharma. Jon developed the world’s first commercially viable 1536-well PCR plates, automated thermal & laser plate-sealer, plus automated liquid-handling & tip washing tools to reduce waste and costs. In 2002 Jon co-founded KBioscience with Phil Robinson, utilising ultra high-throughput PCR instrumentation & a suite of automation tools to create the company’s SNPline robotic platform, with a capacity of 250,000 PCRs/day. The business was underpinned by their ground-breaking patented genotyping chemistry, KASP™, which has over 10,000 scientific papers to date. In November 2022 Jon joined 3CR Bioscience acting as an advisor bringing his commercial and scientific experience to the company.