A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.
With our leadership team’s long history of developing commercial products with substance, we believe 3CR Bioscience is uniquely positioned to facilitate our vision of making science affordable. Our products encompass PCR genotyping, molecular markers, pathogen detection, and more.
We specialise in developing high-performance products for various disciplines including plant, animal, human, and microbial genomics. We work alongside our customers to ensure our precision products are accessible and can be implemented effectively, regardless of budget or experience.
Grow your research, optimise productivity, relieve bottlenecks, and generate outstanding results with 3CR Bioscience. Discover cutting-edge innovation, advanced molecular biology tools, unrivalled technical support, and custom solutions to meet a diverse range of needs.
Unlock the benefits of exceptional quality genotyping reagents, compatible with all allele-specific PCR assays, including PACE®, KASP™ and TaqMan®. Generate consistent, accurate data at any volume, with scalable and cost-effective solutions.
Develop breeder-friendly markers rapidly from genomic variants such as SNPs and InDels with our free PACE assay design service and full or partial assay validation options. Accelerate your work, achieve your goals.
Obtain consistent, accurate genotyping data without sample purification, even from challenging or high-throughput starting material. Streamline your workflow and reduce costs and consumables without compromising data quality.
Customised precision technology for the fast, one-step detection of DNA and RNA-based pathogens. Proven efficacy and sensitivity across agricultural, veterinary, and medical applications.
Revolutionise your PCR genotyping with simultaneous detection of multiple targets in a single reaction. Reduce time, cost, and consumables with increased insight.
Access skills and knowledge that will maximise the rapid production of top-quality data routinely in your facility, regardless of its size or your level of experience.
Just some of the data on our industry leading reagent performance, assay development and customer service record.
Paving the way for protected plant
diversity, sustainable agriculture, and enhanced
crop traits with PACE genotyping
Advancing animal health, breeding, and conservation with PACE genotyping
Genotyping at the frontiers of human health, investigating human genomics and disease
Products:
Our patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It provides consistent performance making it ideal for high-throughput genotyping applications. PACE genotyping master mixes are designed for use with PACE Genotyping Assays and are also compatible with pre-existing KASP™ and Amplifluor® assays
Unlike probe-based products such as TaqMan™, PACE assays only require un-labelled primer oligos to make up the PACE genotyping reaction, in combination with PACE Genotyping Master Mix. Watch our quick video explaining how PACE genotyping works.
All our master mixes are supplied at 2x concentration for convenience and contain ROX normalising dye at either high (500 nM working concentration), low (25 nM working concentration), standard ROX level (150 nM working concentration) or without ROX.
3CR Bioscience provides a free PACE assay design service plus full- or partial assay validation options to help you reduce the cost and turnaround time of your genotyping.
For Research and Development purposes only. Not for diagnostic use.
Legal Information
KASP™ is a trademark of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
TaqMan™ is trademark of Roche Molecular Systems, Inc.
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More Information A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.
An enhanced PCR master mix for allele-specific assays. Improved signal to noise ratio and tight clustering. Developed specifically for genotyping direct from crude DNA samples.
PACE Multiplex Master Mix for the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.
Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
Advanced performance at ultra-low reaction volumes, delivering consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.
A custom designed PACE® Genotyping Assay, designed to your target sequence. Compatible with all PACE genotyping master mixes.
A cost-effective screening solution to detect CRISPR and other gene edits.
Everything you need to run a trial PACE Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.
ProbeSure™ Genotyping Master Mix is designed for use with 5’ nuclease assays and is suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes. ProbeSure Genotyping Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.
ProbeSure Genotyping Master Mix has been carefully optimised to work over a wide dynamic range of reaction volumes and sample template qualities. ProbeSure Genotyping Master Mix combines class-leading performance with the most competitive prices in the market.
For Research and Development purposes only. Not for diagnostic use.
Legal Information
TaqMan™ is a registered trademark of Roche Molecular Systems, Inc.
Zen™ Probe is a trademark of Integrated DNA Technologies, Inc.
KASP™, BHQ™ Probes, and BHQplus™ Probes are trademarks of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.
For the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.
Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
3CR Bioscience has developed precision solutions for rapid pathogen detection, applicable right across agricultural, veterinary, and medical disciplines. We have created PACE and ProbeSure OneStep RT-PCR, fluorescent PCR detection systems that can be customised for any DNA or RNA-based pathogens. Recent examples include the rapid, large-scale detection of agricultural disease outbreaks and the monitoring of circulating SARS-COV-2 variants within a population.
We have worked with many companies and organisations to develop a pathogen detection system and have now scaled-up our development to provide this as a service. Should you be interested in working with us to develop a pathogen detection system, please contact us at support@3crbio.com.
Customer supplies target DNA / RNA sequences. 3CR Bioscience designs assay(s) in-house, and orders primers and gBlocks for validation.
Pre-validated and optimised pathogen detection components are shipped to the customer. Components include validated assay and master mix (including RT enzyme for RNA pathogens) for the customer to test in their facility.
Pre-validated and optimised kit are shipped to the customer. The kit consists of validated assay and master mix (plus RT enzyme for RNA kits) for the customer to test samples on in-house qPCR instrument.
An assay panel is a set of assays that can be supplied to our customers in either validated or non-validated format.
There are several reasons that some of our customers use assay panels, such as:
Should you wish to work with us to develop a specific panel please contact us.
Customers can use our free PACE assay design service to design allele-specific assays for use with our PACE Genotyping Master Mixes. We have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR. We also offer an additional full- or partial in-house assay validation service.
Our in-house scientific team design assays to your target sequences and then provide you with the optimised primer designs and sequences free of charge, allowing you to order from your chosen oligo manufacturer. We are not an oligo manufacturer, so prefer to concentrate on what we’re good at and let the experts manufacture the oligos.
From experience, we highly recommend IDT for oligo / assay synthesis. We have collaborated with IDT for many years and have always found their products to be of the highest quality, and are therefore our oligo / assay manufacturer of choice.
To take advantage of our free PACE assay design service, you must have purchased PACE Genotyping Master Mix within the last 24 months.
For Research and Development purposes only. Not for diagnostic use.
Legal Information
KASP™ is a trademark of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
TaqMan™ is trademark of Roche Molecular Systems, Inc.
Existing PACE Genotyping Master Mix customers can fill in the Customer Information and Customer Sequences pages of the PACE assay design submission template and email it to support@3crbio.com with your sequence(s) and targeted polymorphism(s), and we’ll design assays that are compatible with our PACE Genotyping Master Mixes.
Blank template to be populated with your sequences for which you require assays to be designed.
Details of how to complete the Customer Sequences page.
Detailing examples of how to submit various types of sequence.
Blank template to be populated with your sequences for which you require assays to be designed.
These are required if you have sequences with multiples markers. Download codes here.
Shows the oligo sequences, the allele definitions, Tm (°C) and GC content (%).
Lists the primer IDs and sequences, so that they can be copied and pasted directly into the IDT oligo ordering form to receive assays in plates, should you wish.
It is cheaper to order oligos individually from an oligo company such as IDT than it is to order ready made assays
Our design team has in excess of 100 years experience of designing and optimising primers for use in allele-specific PCR.
With our relationship with Integrated DNA Technologies (IDT) now well established, we continue to provide free PACE assay designs. We now also provide a pre-populated IDT ordering form for you to order your oligos from IDT and to receive the assays with all three oligos ready mixed in a well of a 96-well plate.
Your ideas don’t always require traditional or existing catalogue products. You may require tailored product sizes, bulk product manufacturing, unique formulations or a specific format that is unavailable off-the-shelf. Contact us to discuss your requirements.
We offer our customers a free assay design service for allele-specific primers for use with all PACE Genotyping Master Mixes.
We are a master mix reagent manufacturer and not an oligo manufacturer. We want to concentrate on what we’re good at and let the experts manufacture the oligos. However, we do have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR.
We therefore are happy to offer this expertise to our customers and provide them with primer sequences, which they can then take to their oligo manufacturer of choice for synthesis.
From experience, we highly recommend IDT for oligo/assay synthesis. We have collaborated with IDT for many years and have always found their products to be of the highest quality, so much so, they are our oligo manufacturer of choice.
We listen to and work with our customers to meet their requirements
Customised process, customised quality control
Small to large, we are happy to manage a variety of custom volumes and pack sizes
Flexible order sizes, and custom packaging and labelling available
We listen to and work with our customers to meet their requirements
We are open to OEM opportunities. Get in contact to discuss
We listen and work with our customers to meet their requirements
Customised process, customised quality, control
Small to large, we are happy to manage a variety of volumes custom pack sizes
Flexible order volume, custom packaging and labelling, customer branding
Flexible order volume, custom packaging and labelling, customer branding
We listen and work with our customers to meet their requirements
We listen and work with our customers to meet their requirements
Your ideas don’t always require traditional or existing catalogue products. You may require tailored product sizes, bulk product manufacturing, unique formulations or a specific format that is unavailable off-the-shelf.
Contact us to discuss your requirements.
A selection of high-quality consumables and tools compatible with high-throughput genotyping instruments and automation, with additional items to complement our genotyping lab services.
High-speed whole rack barcode scanner with HD imaging for accurate decoding and automation.
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Disposable polypropylene tip combs for use with our H9600 and H384 DNA extraction instruments.
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Discover our high-quality 96 deep well plates with a 2 ml capacity, designed specifically for extraction robots and automated workflows. These plates ensure reliable sample storage, chemical resistance, and consistent results in every application. Ideal for laboratories handling high-throughput processes, our 96 deep well plates (2 ml) are compatible with most extraction robots.
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Sterile, nuclease-free Racked Sample Tubes with 2D and 3D coding – designed for accurate sample tracking, automated handling, and use in clinical and research settings.
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Our 384-well PCR plates have a rigid fully-skirted frame which remains stable during thermal cycling.
A handheld 8-tube screw cap tube capper/decapper. Securely seal or unscrew an 8-cap row in just 10 seconds, or an entire rack of 96 tubes in 2 minutes.
For simplified collection and shipment of leaf samples for our premium DNA Extraction and Genotyping Lab Services.
Custom low volume, low profile/slim 384-well plates
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Our high-quality PCR plate sealing film ensures secure, airtight heat sealing of 96- and 384-well microplates, preventing evaporation and cross-contamination during thermal cycling.
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Efficient, leaf punch tools for quick, precise field sampling. For use with the 3CR Bio Leaf Sample Collection Kit.
Founded in 2017, 3CR Bioscience Limited is led by a group of scientists who have built close ties together working for 20 years in R&D, product development and manufacturing, and who wanted to make high-quality scientific reagents affordable for all. We share the belief that more affordable reagents will empower scientists to produce
more data, with better outcomes, accelerating all areas of science for positive change.
Discover for yourself why 3CR Bioscience is a trusted partner for breeding programs wordwide, from large seed and livestock producers to individual breeders feeding local communities.
Over 100 years experience in R&D, new product development, manufacturing and quality control.
Extensive quality-control processes.
Quick turnaround times on orders.
Free PACE assay design service for users
Knowledgeable after sales support.
A company forged through decades of high-throughput genotyping innovation, founded and operated by scientists dedicated to serving the scientific commuity.
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