PCR Genotyping

PCR Genotyping Made Simple

PACE® chemistry and user-friendly instruments designed to accelerate your research—accurate, scalable and cost-effective.

Shop Master Mixes
Discover Our Instruments

Trusted by leading research labs and Service Providers

More than 600+ Universities and Businesses trust in us.

ilife-logo-1
Logo_Longping-Hightech
Starke Ayres-logo
Vix Genetics_logo
Covercress logo
NCSU
Logo-Fitó-(Verde)
Julies-kuehn logo
Ramiro Arnedo Logo
Sakata Seed India logo
ICAR-IIHR logo
genetikascience-final-logo-large
Namdhari Seed Pvt Ltd logo
DSV_Logo_rgb
Hyline_logo_vert_low res
UPJV

Making Science AffordablE

With our leadership team’s long history of developing commercial products with substance, we believe 3CR Bioscience is uniquely positioned to facilitate our vision of making science affordable. Our products encompass PCR genotyping, molecular markers, pathogen detection, and more.


We specialise in developing high-performance products for various disciplines including plant, animal, human, and microbial genomics. We work alongside our customers to ensure our precision products are accessible and can be implemented effectively, regardless of budget or experience.

Endpoint genotyping

Improving PCR Genotyping for Your Sector

With decades of experience developing robust, commercially successful molecular tools, our team is dedicated to advancing PCR genotyping across agriculture, aquaculture, livestock and beyond. From molecular markers to pathogen detection, our affordable, high-performance products are designed to support the unique needs of your industry.

Crop

Learn More

Aquaculture

Learn More

Livestock

Learn More

Life Science

Learn More

Fruit

Learn More

Vegetable

Learn More

Accelerate your science

Grow your research, optimise productivity, relieve bottlenecks, and generate outstanding results with 3CR Bioscience. Discover cutting-edge innovation, advanced molecular biology tools, unrivalled technical support, and custom solutions to meet a diverse range of needs.

Technology leading genotyping reagents

Unlock the benefits of exceptional quality genotyping reagents, compatible with all allele-specific PCR assays, including PACE®, KASP™ and TaqMan®. Generate consistent, accurate data at any volume, with scalable and cost-effective solutions.

Take the guesswork out of marker development

Develop breeder-friendly markers rapidly from genomic variants such as SNPs and InDels with our free PACE assay design service and full or partial assay validation options. Accelerate your work, achieve your goals.

High-quality genotyping data, no purification needed

Obtain consistent, accurate genotyping data without sample purification, even from challenging or high-throughput starting material. Streamline your workflow and reduce costs and consumables without compromising data quality.

Rapid, sensitive pathogen detection

Customised precision technology for the fast, one-step detection of DNA and RNA-based pathogens. Proven efficacy and sensitivity across agricultural, veterinary, and medical applications.

MULTIPLEX GENOTYPING - EXPANDING POSSIBILITIES

Revolutionise your PCR genotyping with simultaneous detection of multiple targets in a single reaction. Reduce time, cost, and consumables with increased insight.

Industry leading support at every step

Access skills and knowledge that will maximise the rapid production of top-quality data routinely in your facility, regardless of its size or your level of experience.

USE OUR TECHNOLOGY

Our results in numbers

Just some of the data on our industry leading reagent performance, assay development and customer service record.

PACE genotyping accuracy, independently verified
0 %
YEARS OF COMBINED TECHNICAL EXPERIENCE
0 +
customers worldwide
0 +
assays designed to date
0 Million
seed producers worldwide trust PACE genotyping
0 /TOP 10

Our product portfolio for your PCR genotyping workflow

Our portfolio of products and services include PACE® genotyping chemistry, instruments, and lab services to streamline every step of your workflow. Designed for life sciences, biotech, and agricultural research, our high-performance reagents, reliable instruments, and expert lab support help you achieve accurate, consistent results while reducing time and costs – making science affordable.

PACE® 2x Genotyping Master Mix for high-accuracy SNP genotyping and allele-specific PCR

PRODUCTS

Technician installing multi-channel pipetting head in automated liquid handling system

Instrumentation

Wheat ears in field representing crop breeding and agricultural genomics

Marker Panels

DNA extraction lab

Lab Services

what our clients say about us

“Thank you so much for being such a great company to work with. Your customer service has been wonderful. We are looking forward to developing more PACE markers linked to other disease resistant genes. Your PACE 2.0 Master Mix has worked beautifully. 3CR’s generosity to design PACE primers around SNPs is greatly appreciated. Thank you again.”

David C. Zlesak, Ph.D.

Professor of Horticulture, UWRF Plant and Earth Science Department

“I am very glad to see the level of support you provided on this troubleshooting, and I was happy to see such feedback from Marin who has years of experience with this assay – kudos to your team at 3CR!”

Director of R&D

Industry-leading Laboratory 
Service Provider

“Over the course of several years, we have consistently experienced exceptional delivery times and communication from 3CR. The level of service they provide not only meets but greatly facilitates our global ambitions. Their commitment to excellence has proven to be a valuable asset, contributing significantly to the efficiency and success of our operations on a global scale.”

DKSH

“We have used PACE since modernizing our breeding program back in 2018. It is affordable, simple to use, and has never failed us! We constantly recommend it to others looking to expand their genotyping and marker-assisted selections programs!”

North Carolina State University

Peanut Breeding and Genetics program

“As a newly established company, finding cost-effective and approachable partners is essential. 3CR BIO has proven to be exactly that; offering competitive pricing without compromising on the quality of their products and services. Beyond their professionalism, I had the pleasure of meeting the team in person and can confidently say they are not only skilled but truly great people to work with. Whether you’re a large company or just starting out, 3CR BIO treats you with the same level of care and commitment’”

“The team at 3CR have been so thorough and helpful at troubleshooting even small genotyping irregularities.

PACE Nano is an exceptional product, massively improving data quality from difficult sample types or challenging assays.

We’ve enjoyed working with 3CR – they are efficient, responsive to our needs, and their products perform as promised.’”

“For a small horticultural seed company like ours that is in the process of modernizing its genotyping lab, we highly recommend working with 3CR’s professional team. We very much appreciate the accessibility and scientific expertise in the realm of molecular genetics in general and genotyping in particular to support us in reaching our breeding objectives as fast and efficiently as possible.”

“Working with 3CR has been remarkably smooth and supportive – their technology exceeded expectations, and their team truly understands the meaning of trust and long-term partnership.”

“3CR Biosciences delivers unmatched reliability and innovation in the field of agriculture research. They have strong technical and customer centric team. As their partners in India, iLife Biotech had an exciting journey to support scientists with fast, flexible and cost effective genotyping tools. The PACE assays has redesigned the genotyping workflows in the Indian Seed Companies.”

“We are very happy and super satisfied with the performance of PACE Genotyping. Our customers love PACE very much too! Also, the cost is very effective making PACE the ultimate genotyping solution especially for our agrigenomic customers in Indonesia.”

genetikascience-final-logo-large

“The creativity in overcoming genotyping challenges has been instrumental to the success of the research and development function.”

“PACE Genotyping Mastermix has significantly improved the throughput and accuracy of our SNP genotyping projects. The mix is incredibly robust and easy to use, even with challenging DNA samples. We’ve reduced our reaction setup time and seen consistent results across hundreds of samples. It’s now our go-to solution for high-throughput genotyping.”

Mr.Ranjan Shaw

Biotechnology R&D, Namdhari Seed pvt ltd

Namdhari Seed Pvt Ltd logo

“In our Biotechnology research lab, we’ve adopted PACE Genotyping Mastermix for SNP analysis across various plant and crops. The mix has proven to be highly reliable, delivering consistent and reproducible results even when working with low-concentration or partially degraded DNA samples. The simplicity of the protocol has also reduced hands-on time and minimized error rates during large-scale genotyping projects. It’s become a key component of our molecular biology workflow and has greatly supported our publication-quality data generation.”

Dr. Lakshmana Reddy Dc

Principal Scientist, ICAR-Indian Institute

“We are using PACE mastermix in our routine genotyping work. PACE master mix with features like work with crude DNA, tight and clear clusters on plot, cost effectiveness and versatile in nature makes excellent product to use.”

Mr.Vinay Jain

Scientist (Molecular Biology-R&D)

“We’re very satisfied with the quality and reliability of the genotyping solutions provided by 3CR, but above all, we truly value the close support and responsiveness the team has offered over the years.”

“My lab has relied on the 3CR PACE mastermix for years, and it has consistently delivered reliable, reproducible results. As a laboratory manager, I value how 3CR’s products and instrumentation help reduce per-sample costs, increase throughput, and accelerate assay development. Their exceptional support team and engineering innovations ensure low operating costs and minimal downtime, making them a vital partner in our workflow.”

“3CR Bioscience Limited provides us with DNA extracted from thousands of freeze-dried wheat seeds (we send in 96-deep well plates), offering a reliable and high-quality service.

DNA quality and quantity are perfectly suitable for further SNPs genotyping we perform by qPCR.

The support team is very efficient with easy communication. The delivery of the DNA samples is fast and safe, using robust packing.

Service highly recommended!”

Emilie JACOB (CAVEL)

3CR Blog

Explore our blog for regular tech notes, insightful articles on the latest research, application notes, and compelling case studies highlighting the effectiveness of our genotyping products and services.

See All Blog Posts
TURBO GRINDER USER GUIDE
Learn how to install, configure, operate and maintain the Turbo Grinder with step-by-step instructions, software setup, troubleshooting tips and best practices for reliable high-throughput sample homogenisation.
PACE® GENOTYPING MASTER MIX – USER GUIDE
Get everything you need to run successful PACE® genotyping reactions, from DNA and assay setup to thermal cycling, fluorescence detection and genotype interpretation.
PACE® NANO GENOTYPING MASTER MIX TRIAL KIT USER GUIDE
Everything you need to get started with PACE® Nano Genotyping—from reaction setup and ROX compatibility to endpoint detection and data interpretation—in one practical user guide.
EU Approves New Genomic Techniques Regulation: What It Means for Plant Breeding and High-Throughput Genotyping
Discover how the EU's new NGT regulation is reshaping plant breeding and why scalable genotyping is becoming essential for genome editing success.
PACE® Genotyping and New Advances in Waxy Sorghum Breeding
PACE® Genotyping Drives Precision Breeding in Cranberry’s

3CR News

Discover the latest updates and upcoming events from 3CR Bioscience, along with key developments from our valued partners, MolBreeding Biotech and HC Scientific.

See All News Posts
[fibosearch]

Quick Links:

Documentation
PACE User Manual
PACE Certificate
← Back to log in
This field is for validation purposes and should be left unchanged.
Name(Required)
Consent(Required)

New customer &#8594

Contact us

clear
SEND

Resolve Genotyping Challenges. With One Practical Guide.

From failed amplification to scattered cluster plots — this guide provides structured, expert-backed solutions for the 7 most common PACE® genotyping issues.
get more information

"(Required)" indicates required fields

This field is for validation purposes and should be left unchanged.
I’d like to sign up for the newsletter
Consent to privacy policy(Required)

MEET OUR TEAM

Steve AsquithManaging Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John HolmeTechnical Director

John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.

During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.

Dr. Nisha JainOperations Director

Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.

Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.

Nazma SaffinGeneral Manager

For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.

Greig PollandAutomation and Support Manager

Greig is a hands-on automation specialist and team leader with a strong background in laboratory and industrial automation. He has spent over 25 years developing, installing, and supporting automated systems that transformed laboratory workflows. During this time, Greig worked closely with scientists and engineers to tailor automation solutions for genotyping and molecular biology, an experience that sparked his lasting passion for combining technology with practical science.

Since then, Greig has built on that foundation through leadership roles where he leads automation and support operations. He’s known for being approachable, commercially minded, and deeply committed to helping teams and customers get the best from their technology.

Whether managing a complex automation rollout or helping a customer troubleshoot in real time, Greig brings a thoughtful, collaborative approach that keeps people ,not just machines, at the centre of what he does.

Daniel LawsonMarketing Manager
Daniel is a strategically minded marketing and communications professional with over a decade of experience delivering integrated, multi-channel campaigns across healthcare, legal and not-for-profit sectors. He has a strong track record in building brand visibility, driving engagement and supporting revenue growth through audience-focused marketing.
Prior to joining 3CR Bioscience in 2025, Daniel held senior marketing roles where he led strategic communications, managed brand development and delivered high-impact digital and content campaigns. His experience spans SEO, CRM, PR, events and stakeholder engagement, with a particular strength in translating complex messages into clear, compelling content.
At 3CR Bioscience, Daniel is responsible for shaping and executing the company’s marketing strategy, enhancing brand presence and supporting commercial growth through targeted, data-driven campaigns.