Our patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It provides consistent performance making it ideal for high-throughput genotyping applications. PACE genotyping master mixes are designed for use with PACE Genotyping Assays and are also compatible with pre-existing KASP™ and Amplifluor® assays
Unlike probe-based products such as TaqMan™, PACE assays only require un-labelled primer oligos to make up the PACE genotyping reaction, in combination with PACE Genotyping Master Mix. Watch our quick video explaining how PACE genotyping works.
All our master mixes are supplied at 2x concentration for convenience and contain ROX normalising dye at either high (500 nM working concentration), low (25 nM working concentration), standard ROX level (150 nM working concentration) or without ROX.
3CR Bioscience provides a free PACE assay design service plus full- or partial assay validation options to help you reduce the cost and turnaround time of your genotyping.
For Research and Development purposes only. Not for diagnostic use.
Legal Information
KASP™ is a trademark of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
TaqMan™ is trademark of Roche Molecular Systems, Inc.
A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.
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An enhanced PCR master mix for allele-specific assays. Improved signal to noise ratio and tight clustering. Developed specifically for genotyping direct from crude DNA samples.
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PACE Multiplex Master Mix for the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.
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Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
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Advanced performance at ultra-low reaction volumes, delivering consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.
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A custom designed PACE® Genotyping Assay, designed to your target sequence. Compatible with all PACE genotyping master mixes.
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Everything you need to run a trial PACE Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.
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ProbeSure™ Genotyping Master Mix is designed for use with 5’ nuclease assays and is suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes. ProbeSure Genotyping Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.
ProbeSure Genotyping Master Mix has been carefully optimised to work over a wide dynamic range of reaction volumes and sample template qualities. ProbeSure Genotyping Master Mix combines class-leading performance with the most competitive prices in the market.
For Research and Development purposes only. Not for diagnostic use.
Legal Information
TaqMan™ is a registered trademark of Roche Molecular Systems, Inc.
Zen™ Probe is a trademark of Integrated DNA Technologies, Inc.
KASP™, BHQ™ Probes, and BHQplus™ Probes are trademarks of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.
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For the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.
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Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
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3CR Bioscience has developed precision solutions for rapid pathogen detection, applicable right across agricultural, veterinary, and medical disciplines. We have created PACE and ProbeSure OneStep RT-PCR, fluorescent PCR detection systems that can be customised for any DNA or RNA-based pathogens. Recent examples include the rapid, large-scale detection of agricultural disease outbreaks and the monitoring of circulating SARS-COV-2 variants within a population.
We have worked with many companies and organisations to develop a pathogen detection system and have now scaled-up our development to provide this as a service. Should you be interested in working with us to develop a pathogen detection system, please contact us at support@3crbio.com.
Customer supplies target DNA / RNA sequences. 3CR Bioscience designs assay(s) in-house, and orders primers and gBlocks for validation.
Pre-validated and optimised pathogen detection components are shipped to the customer. Components include validated assay and master mix (including RT enzyme for RNA pathogens) for the customer to test in their facility.
Pre-validated and optimised kit are shipped to the customer. The kit consists of validated assay and master mix (plus RT enzyme for RNA kits) for the customer to test samples on in-house qPCR instrument.
Customers can use our free PACE assay design service to design allele-specific assays for use with our PACE Genotyping Master Mixes. We have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR. We also offer an additional full- or partial in-house assay validation service.
Our in-house scientific team design assays to your target sequences and then provide you with the optimised primer designs and sequences free of charge, allowing you to order from your chosen oligo manufacturer. We are not an oligo manufacturer, so prefer to concentrate on what we’re good at and let the experts manufacture the oligos.
From experience, we highly recommend IDT for oligo / assay synthesis. We have collaborated with IDT for many years and have always found their products to be of the highest quality, and are therefore our oligo / assay manufacturer of choice.
To take advantage of our free PACE assay design service, you must have purchased PACE Genotyping Master Mix within the last 24 months.
For Research and Development purposes only. Not for diagnostic use.
Legal Information
KASP™ is a trademark of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
TaqMan™ is trademark of Roche Molecular Systems, Inc.
Existing PACE Genotyping Master Mix customers can fill in the Customer Information and Customer Sequences pages of the PACE assay design submission template and email it to support@3crbio.com with your sequence(s) and targeted polymorphism(s), and we’ll design assays that are compatible with our PACE Genotyping Master Mixes.
Blank template to be populated with your sequences for which you require assays to be designed.
Details of how to complete the Customer Sequences page.
Detailing examples of how to submit various types of sequence.
Blank template to be populated with your sequences for which you require assays to be designed.
These are required if you have sequences with multiples markers. Download codes here.
Shows the oligo sequences, the allele definitions, Tm (°C) and GC content (%).
Lists the primer IDs and sequences, so that they can be copied and pasted directly into the IDT oligo ordering form to receive assays in plates, should you wish.
It is cheaper to order oligos individually from an oligo company such as IDT than it is to order ready made assays
Our design team has in excess of 100 years experience of designing and optimising primers for use in allele-specific PCR.
With our relationship with Integrated DNA Technologies (IDT) now well established, we continue to provide free PACE assay designs. We now also provide a pre-populated IDT ordering form for you to order your oligos from IDT and to receive the assays with all three oligos ready mixed in a well of a 96-well plate.
Your ideas don’t always require traditional or existing catalogue products. You may require tailored product sizes, bulk product manufacturing, unique formulations or a specific format that is unavailable off-the-shelf. Contact us to discuss your requirements.
We offer our customers a free assay design service for allele-specific primers for use with all PACE Genotyping Master Mixes.
We are a master mix reagent manufacturer and not an oligo manufacturer. We want to concentrate on what we’re good at and let the experts manufacture the oligos. However, we do have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR.
We therefore are happy to offer this expertise to our customers and provide them with primer sequences, which they can then take to their oligo manufacturer of choice for synthesis.
From experience, we highly recommend IDT for oligo/assay synthesis. We have collaborated with IDT for many years and have always found their products to be of the highest quality, so much so, they are our oligo manufacturer of choice.
We listen to and work with our customers to meet their requirements
Customised process, customised quality control
Small to large, we are happy to manage a variety of custom volumes and pack sizes
Flexible order sizes, and custom packaging and labelling available
We listen to and work with our customers to meet their requirements
We are open to OEM opportunities. Get in contact to discuss
We listen and work with our customers to meet their requirements
Customised process, customised quality, control
Small to large, we are happy to manage a variety of volumes custom pack sizes
Flexible order volume, custom packaging and labelling, customer branding
Flexible order volume, custom packaging and labelling, customer branding
We listen and work with our customers to meet their requirements
We listen and work with our customers to meet their requirements
Your ideas don’t always require traditional or existing catalogue products. You may require tailored product sizes, bulk product manufacturing, unique formulations or a specific format that is unavailable off-the-shelf.
Contact us to discuss your requirements.
A selection of high-quality consumables and tools compatible with high-throughput genotyping instruments and automation, with additional items to complement our genotyping lab services.
High-speed whole rack barcode scanner with HD imaging for accurate decoding and automation.
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Disposable polypropylene tip combs for use with our H9600 and H384 DNA extraction instruments.
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2.2mL 96 Deep-Well Plates offer exceptional performance in DNA purification and isolation workflows.
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Compact, Crew Cap, 2D and 3D Coded Sample Tubes, Sterile & Nuclease free
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Our 384-well PCR plates have a rigid fully-skirted frame which remains stable during thermal cycling.
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A handheld 8-tube screw cap tube capper/decapper. Securely seal or unscrew an 8-cap row in just 10 seconds, or an entire rack of 96 tubes in 2 minutes.
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For simplified collection and shipment of leaf samples for our premium DNA Extraction and Genotyping Lab Services.
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Custom low volume, low profile/slim 384-well plates
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Heat sealing film for 96- and 384-well PCR microplates
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Efficient, leaf punch tools for quick, precise field sampling. For use with the 3CR Bio Leaf Sample Collection Kit.
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