PACE Multiplex Master Mix is a cutting-edge, versatile extension of our proven PACE 2.0 Genotyping Master Mix. Specially formulated for simultaneous detection of up to four targets in a single PCR reaction well, this multiplex master mix enables high-throughput analysis, ideal for applications such as genotyping two bi-allelic SNPs or analyzing a reference gene together with three additional genes of interest. It is optimised for use in high-throughput PCR workflows, allowing large sample volumes and multiplexed targets to be processed efficiently and reliably.
PACE Multiplex Master Mix is fully compatible with most qPCR machines and plate readers capable of detecting FAM, HEX, ATTO 590, ATTO 647N, and the ATTO 680 reference dye (please refer to the PACE Multiplex Master Mix User Guide for exact wavelengths). The master mix is supplied at a convenient 2x concentration, with or without ATTO 680 reference dye, and available at various reference dye concentrations for maximum compatibility with your existing workflows.
With PACE Multiplex Master Mix, you can streamline your multiplex PCR assays and boost your genotyping productivity. Experience accurate, reproducible, and cost-effective results for a wide range of applications in genomics, breeding programs, molecular diagnostics, and more, only from 3CR Bioscience.
PACE and PACE 2.0 Genotyping Master Mix can be stored at 4°C for up to two weeks. However, for longer term storage we recommend storing the master mix at -20°C in smaller aliquots to minimise freeze/thaw cycles.
You will require a plate reader capable of reading FAM, HEX, ATTO 590, ATTO 647N and ATTO 680 (the wavelengths of which can be found in our PACE Multiplex Master Mix User Guide). It is important that filters used in the plate reader have narrow bandwidths (10 nm) to avoid cross fluorescence. Alternatively, plate readers using monochromators capable of using at least five channels with 10 nm bandwidth will also work.
PACE Multiplex Master Mix contains uses the fluorophores FAM, HEX, ATTO 590, ATTO 647N. You can use PACE Multiplex Master Mix on any qPCR instrument or with any plate reader that can detect these fluorophores. Please refer to the PACE Multiplex Master Mix User Guide and your instrument manufacturer first.
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John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
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