PACE Multiplex Master Mix is an advanced and versatile extension of our PACE 2.0 Genotyping Master Mix, formulated for the simultaneous detection of up to four targets in one reaction well. For example, two bi-allelic SNPs, or one reference gene and a further three genes of interest.
PACE Multiplex genotyping assay designs are available from 3CR Bioscience through our free PACE assay design service; once designed, users can purchase assay primers independently or through 3CR Bioscience using our partial or full-assay validation service.
Users will require a plate reader capable of reading FAM, HEX, ATTO 550, ATTO 647N and ROX (wavelengths in the PACE Multiplex Master Mix User Guide). PACE Multiplex Master Mix is supplied at 2x concentration for convenience and with ROX normalising dye at a range of levels to ensure compatibility with your qPCR machine or reader.
PACE and PACE 2.0 Genotyping Master Mix can be stored at 4°C for up to two weeks. However, for longer term storage we recommend storing the master mix at -20°C in smaller aliquots to minimise freeze / thaw cycles.
You will require a plate reader capable of reading FAM, HEX, ATTO 550, ATTO 647N and ROX (the wavelengths of which can be found in our PACE Multiplex Master Mix User Guide). It is important that filters used in the plate reader have narrow bandwidths (10 nm) to avoid cross fluorescence. Alternatively, plate readers using monochromators capable of using at least five channels with 10 nm bandwidth will also work.
PACE Multiplex Master Mix contains uses the fluorophores FAM, HEX, ATTO 550, ATTO 647N. You can use PACE Multiplex Master Mix on any qPCR instrument or with any plate reader that can detect these fluorophores. Please refer to the PACE Multiplex Master Mix User Guide and your instrument manufacturer first.
No. However, 5’ nuclease assays are available from a range of manufacturers, such as IDT.