The Mag Plant Pro Genomic DNA Extraction Kit isolates high-quality DNA from a wide range of plant tissues, delivering excellent yields and purity for reliable downstream applications. The extraction method is based on the selective binding of DNA molecules to magnetic beads under specific buffer conditions, followed by separation and purification using a magnetic field – a process known as solid phase extraction.
The Mag Plant Pro Kit includes six reagents designed for efficient, selective DNA extraction:
This kit is optimised for a wide range of plants, including those rich in secondary metabolites (polyphenols, polysaccharides, lipids). Protocol adjustments are provided in the User Guide to maximize yield and purity. For best results, use fresh or young tissue, finely homogenized with liquid nitrogen grinding or mechanical disruption before lysis.
Available in three kit sizes:
The Mag Plant Pro Kit is offered in Small, Medium, and Large versions, each using the same optimized protocol but differing in total DNA yield. As a general guide, the Small Kit yields about 0.5 µg, the Medium Kit yields 1–1.5 µg, and the Large Kit yields ≥2 µg of purified DNA (actual yields vary by seed type and quality). Choose the version that best matches your downstream application and desired DNA output.
Yes. Optimization may be needed for species with challenging chemistries (high secondary metabolites, polysaccharides, or tough cell walls). The User Guide provides specific recommendations, such as adding DTT or β-mercaptoethanol, using liquid nitrogen grinding, or adding carrier RNA.
Yes, with optimizations (e.g., extended lysis, carrier RNA, proteinase K, concentrated elution). Note that degraded DNA may limit downstream applications.
No. The kit can be used with the MagC 9600 Automated Nucleic Acid Extraction System (or similar platforms) following the step-by-step Automated Extraction Protocol provided, or with the Manual Protocol for bench-scale applications.
Freeze at –80 °C. Dried materials should be processed within 1 month.
Standard protocols yield 10–30 kb fragments. For long-read sequencing (≥50 kb), additional specialized methods are recommended.
Up to 100 mg fresh or 30 mg dried tissue.
DNA binds selectively to the surface of magnetic beads under specific buffer conditions. A magnetic field is then used to separate and purify the DNA (solid-phase extraction).
Typically 50 mg fresh or 20 mg dried leaf tissue. This can be adjusted depending on species.
RNA removal depends on tissue type. For complete removal, RNase A can be added (10 µL RNase A per 600 µL Buffer PLS).
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John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
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