Hydrolysis probe PCR mix for TaqMan™, BHQ®, BHQplus®, and ZEN™ assays. Offers accurate, robust performance at competitive pricing.
ProbeSure™ Master Mixes are designed for use with 5’ nuclease assays and are suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes excellent, cost-effective performance.
ProbeSure™ master mixes are designed for use with 5’ nuclease assays and are suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes. ProbeSure Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.
ProbeSure master mixes have been carefully optimised to work over a wide dynamic range of reaction volumes and sample template qualities. ProbeSure master mixes combine class-leading performance with the most competitive prices in the market.
ProbeSure Master Mix is designed for use in PCR applications with hydrolysis probe-based assays with either endpoint or real-time detection. It is formulated for detection of Single Nucleotide Polymorphisms (SNPs) and insertion/deletions (Indels), or detection of sequences of interest. ProbeSure Master Mix can be used with a variety of fluorogenic probe chemistries. It is not intended for use as a master mix for general PCR applications nor for HRM applications. ProbeSure Master Mix contains all the necessary components to carry out the reaction (except template DNA and primers/probes) and is supplied at 2x concentration.
REQUIRED COMPONENTS
Hydrolysis probe PCR mix for TaqMan™, BHQ®, BHQplus®, and ZEN™ assays. Offers accurate, robust performance at competitive pricing.
Simultaneously detect up to four targets per reaction. Streamline workflows, reduce reagent use, and maximise assay data generation.
Perform RNA reverse transcription and PCR in one step. Fast, efficient, and precise RT-PCR for probe-based genotyping.
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John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
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