ProbeSure™

ProbeSure™ Master Mixes are designed for use with 5’ nuclease assays and are suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes excellent, cost-effective performance.

The ProbeSure™ Master Mix Range

ProbeSure™ master mixes are designed for use with 5’ nuclease assays and are suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes. ProbeSure Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.

ProbeSure master mixes have been carefully optimised to work over a wide dynamic range of reaction volumes and sample template qualities. ProbeSure master mixes combine class-leading performance with the most competitive prices in the market.

ProbeSure laboratory

need support in selecting the right MasterMix?

Discover how ProbeSure and 3CR Bio can improve your genotyping workflow. Contact us for help choosing the right product.

Name
This field is for validation purposes and should be left unchanged.

WHY CHOOSE ProbeSure™?

  • Cost-Effective – Reduce genotyping costs by up to 50%
  • High Performance – Reliable results across different reaction volumes and platforms
  • Seamless Compatibility – Suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes
  • Expert Support – Backed by 20+ years of genotyping expertise
  • Quality Assured – Rigorous quality control on every batch

About PROBESURE™

ProbeSure Master Mix is designed for use in PCR applications with hydrolysis probe-based assays with either endpoint or real-time detection. It is formulated for detection of Single Nucleotide Polymorphisms (SNPs) and insertion/deletions (Indels), or detection of sequences of interest. ProbeSure Master Mix can be used with a variety of fluorogenic probe chemistries. It is not intended for use as a master mix for general PCR applications nor for HRM applications. ProbeSure Master Mix contains all the necessary components to carry out the reaction (except template DNA and primers/probes) and is supplied at 2x concentration.

REQUIRED COMPONENTS

  • Fluorescent plate reader or qPCR instrument capable of reading the fluorophores used in the intended assay.
  • PCR plate or equivalent and appropriate optically clear seal
  • PCR-grade water
  • Primers and probes (or both combined into an assay)
  • Template DNA
ProbeSure Master Mix
3CR Bioscience Logo

David C. Zlesak, Ph.D.
Professor of Horticulture, UWRF Plant and Earth Science Department

"Thank you so much for being such a great company to work with. Your customer service has been wonderful. We are looking forward to developing more PACE markers linked to other disease resistant genes. Your PACE 2.0 Master Mix has worked beautifully. 3CR’s generosity to design PACE primers around SNPs is greatly appreciated. Thank you again."

Director of R&D.
Industry-leading Laboratory Service Provider

"I am very glad to see the level of support you provided on this troubleshooting, and I was happy to see such feedback from Marin who has years of experience with this assay - kudos to your team at 3CR!"

DKSH

"As a prominent player in the global life sciences industry, our longstanding collaboration with 3cr Bioscience has been instrumental to our success. Over the course of several years, we have consistently experienced exceptional delivery times and communication from their team. The level of service they provide not only meets but greatly facilitates our global ambitions. Their commitment to excellence has proven to be a valuable asset, contributing significantly to the efficiency and success of our operations on a global scale."

All Products

ProbeSure Genotyping master mix

Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.

ProbeSure Multiplex master mix

For the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.

ProbeSure OneStep RT-PCR Master Mix bottles 200mL, 25mL, 10mL and 2.5mL Low Rox bottles. Available in High Rox, Low ROX, and No Rox formulation,

Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.

Don’t know which one to get?

Unsure which mix to choose? Email us for help choosing the right mix for your application

still Have questions? don’t hesitate to Send us a message.

Send us a message:

This field is for validation purposes and should be left unchanged.

[fibosearch]
Name(Required)
This field is for validation purposes and should be left unchanged.

Contact us

MEET OUR TEAM

Steve Asquith Managing Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John Holme Technical Director
John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base. During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Dr. Nisha JainOperations Director
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP. Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nazma Saffin General Manager
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Jon Curtis Non-Executive Chair
After 8 years in The Royal Air Force, Jon moved to the Imperial Cancer Research Fund where he pioneered the use of ultra high-throughput genomic automation, capable of 46,000 PCRs per hour. In the 1990’s Jon joined GlaxoSmithKline, implementing a high-throughput genomics platform into their drug discovery pipeline. Whilst there he also developed acoustic mixing into compound management, becoming the gold standard across pharma. Jon developed the world’s first commercially viable 1536-well PCR plates, automated thermal & laser plate-sealer, plus automated liquid-handling & tip washing tools to reduce waste and costs. In 2002 Jon co-founded KBioscience with Phil Robinson, utilising ultra high-throughput PCR instrumentation & a suite of automation tools to create the company’s SNPline robotic platform, with a capacity of 250,000 PCRs/day. The business was underpinned by their ground-breaking patented genotyping chemistry, KASP™, which has over 10,000 scientific papers to date. In November 2022 Jon joined 3CR Bioscience acting as an advisor bringing his commercial and scientific experience to the company.