ProbeSure Multiplex Master Mix is an enhanced version of ProbeSure Master Mix, formulated to enable users to analyse up to four targets in one reaction well. For example, two bi-allelic SNPs or one reference gene and a further three genes of interest.
Users will require a plate reader capable of reading FAM, HEX, ATTO 550, ATTO 647N and ATTO 633 (the wavelengths of each of these can be found in our ProbeSure Multiplex Master Mix User Guide). ProbeSure Multiplex Master Mix is supplied at 2x concentration for convenience and is supplied with the ATTO 633 normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ATTO 633.
Any dual-labelled probe with a fluorescent label at the 5′ end and a quencher at the 3′ end, such as TaqMan™, ZEN™ probes and BHQ® and BHQplus® probes, can all be used with ProbeSure Genotyping Master Mix.
We have done no work as yet with these chemistries in digital or other microfluidic systems and it is possible that a small buffer reformulation might be necessary for some platforms. If this is of particular interest to you, please contact us to discuss.