Universal PCR mix for allele-specific assays. Delivers precise fluorescence, high performance, and consistent genotyping across reaction volumes.
PACE® is our patented allele-specific genotyping chemistry, designed for high-throughput PCR applications. PACE master mixes offers consistent, affordable PCR performance and full compatibility with PACE, KASP™ and Amplifluor® assays.
Universal PCR mix for allele-specific assays. Delivers precise fluorescence, high performance, and consistent genotyping across reaction volumes.
Next-generation allele-specific PCR mix with enhanced signal clarity and robust performance, even from crude DNA samples.
Detect up to four targets in one PCR reaction. Save time, reduce consumables, and maximise throughput with effortless multiplexing.
Our patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It provides consistent performance making it ideal for high-throughput genotyping applications. Its robust chemistry is especially well-suited for high-throughput PCR workflows, where reliable allele discrimination is essential at scale. PACE genotyping master mixes are designed for use with PACE Genotyping Assays and are also compatible with pre-existing KASP™ and Amplifluor® assays.
Unlike probe-based products such as TaqMan™, PACE assays only require un-labelled primer oligos to make up the PACE genotyping reaction, in combination with PACE Genotyping Master Mix. Watch our quick video explaining how PACE genotyping works.
All our master mixes are supplied at 2x concentration for convenience and contain ROX normalising dye at either high (500 nM working concentration), low (25 nM working concentration), standard ROX level (150 nM working concentration) or without ROX.
Boost Your Genotyping Workflows with PACE® Multiplex!
Looking to double your data output while cutting down on DNA, consumables, and labour?
PACE® Multiplex Master Mix makes it possible—no optimisation required!
Customers can use our free PACE assay design service to design allele-specific assays for use with our PACE Genotyping Master Mixes. We have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR. We also offer an additional full- or partial in-house assay validation service.
Our in-house scientific team design assays to your target sequences and then provide you with the optimised primer designs and sequences free of charge, allowing you to order from your chosen oligo manufacturer. We are not an oligo manufacturer, so prefer to concentrate on what we’re good at and let the experts manufacture the oligos.
From experience, we highly recommend IDT for oligo / assay synthesis. We have collaborated with IDT for many years and have always found their products to be of the highest quality, and are therefore our oligo / assay manufacturer of choice.
During assay optimisation, certain SNP or Indel targets may benefit from a touchdown PCR protocol to enhance specificity and yield. Our technical team can provide guidance on when and how to apply this approach for optimal PACE® performance.
To take advantage of our free PACE assay design service, you must have purchased PACE Genotyping Master Mix within the last 24 months.
PACE® PCR technology is ideal for high-throughput SNP genotyping across a wide range of species and research areas.
Our allele-specific PCR chemistry enables precise discrimination of single nucleotide polymorphisms (SNPs) and small insertions or deletions, delivering robust results even from crude DNA extracts.
Whether you’re validating marker-trait associations, supporting crop and livestock breeding, or conducting population genetics research, PACE reagents simplify genotyping SNPs with accuracy, scalability, and cost-efficiency.
Universal PCR mix for allele-specific assays. Delivers precise fluorescence, high performance, and consistent genotyping across reaction volumes.
Next-generation allele-specific PCR mix with enhanced signal clarity and robust performance, even from crude DNA samples.
Detect up to four targets in one PCR reaction. Save time, reduce consumables, and maximise throughput with effortless multiplexing.
Genotype directly from RNA. Combines reverse transcription and PCR amplification in a single, streamlined reaction.
High-performance, ultra-low-volume PCR mix for cost-effective, high-throughput genotyping in automated or miniaturised workflows.
Fast, affordable PACE®-based screening to detect CRISPR and gene edits with high accuracy in high-throughput applications.
Custom PACE® assays designed for your target sequence. Ensure reliable, accurate allele detection with full PACE® compatibility.
All-in-one kit to trial PACE® genotyping. Includes assays, master mix, control DNA, and a detailed setup guide.
Unsure which mix to choose? Email us for help choosing the right mix for your application
No FAQs found.
Sign up now to receive insights, updates and industry case studies.
John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
You are currently viewing a placeholder content from Facebook. To access the actual content, click the button below. Please note that doing so will share data with third-party providers.
More InformationYou are currently viewing a placeholder content from X. To access the actual content, click the button below. Please note that doing so will share data with third-party providers.
More Information