A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.
PACE® is our patented allele-specific genotyping chemistry, designed for high-throughput PCR applications. PACE master mixes offers consistent, affordable PCR performance and full compatibility with PACE, KASP™ and Amplifluor® assays.
Our patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It provides consistent performance making it ideal for high-throughput genotyping applications. PACE genotyping master mixes are designed for use with PACE Genotyping Assays and are also compatible with pre-existing KASP™ and Amplifluor® assays.
Unlike probe-based products such as TaqMan™, PACE assays only require un-labelled primer oligos to make up the PACE genotyping reaction, in combination with PACE Genotyping Master Mix. Watch our quick video explaining how PACE genotyping works.
All our master mixes are supplied at 2x concentration for convenience and contain ROX normalising dye at either high (500 nM working concentration), low (25 nM working concentration), standard ROX level (150 nM working concentration) or without ROX.
Boost Your Genotyping Workflows with PACE® Multiplex!
Looking to double your data output while cutting down on DNA, consumables, and labour?
PACE® Multiplex Master Mix makes it possible—no optimisation required!
Discover how PACE and 3CR Bio can improve your genotyping workflow. Contact us for help choosing the right product or a free trial of the PACE Genotyping Master Mix.
Customers can use our free PACE assay design service to design allele-specific assays for use with our PACE Genotyping Master Mixes. We have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR. We also offer an additional full- or partial in-house assay validation service.
Our in-house scientific team design assays to your target sequences and then provide you with the optimised primer designs and sequences free of charge, allowing you to order from your chosen oligo manufacturer. We are not an oligo manufacturer, so prefer to concentrate on what we’re good at and let the experts manufacture the oligos.
From experience, we highly recommend IDT for oligo / assay synthesis. We have collaborated with IDT for many years and have always found their products to be of the highest quality, and are therefore our oligo / assay manufacturer of choice.
To take advantage of our free PACE assay design service, you must have purchased PACE Genotyping Master Mix within the last 24 months.
A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.
An enhanced PCR master mix for allele-specific assays. Improved signal to noise ratio and tight clustering. Developed specifically for genotyping direct from crude DNA samples.
PACE Multiplex Master Mix for the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.
Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
Advanced performance at ultra-low reaction volumes, delivering consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.
A cost-effective screening solution to detect CRISPR and other gene edits.
A custom designed PACE® Genotyping Assay, designed to your target sequence. Compatible with all PACE genotyping master mixes.
Everything you need to run a trial PACE Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.
Unsure which mix to choose? Email us for help choosing the right mix for your application
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