Our custom CRISPR detection assays are designed to identify your specific genome edits whether introduced via directed CRISPR editing or Non-Homologous End Joining (NHEJ) using DNA sequences you provide, including the target site, spacer, and PAM. Simply submit your assay details using our provided template and guidelines.
Traditional high-throughput sequencing can be time-consuming and expensive. The PACE® CRISPR detec tion assay offers a rapid, scalable, and cost-effective solution for primary screening of gene edits, making it ideal for both agricultural and life sciences research.
Confidentiality Assured: Each client’s assay primers are proprietary and confidential. Every order includes 400 µL of ready-to-use 72x PACE assay, enough for at least 2,800 reactions at a 10 µL volume.
How does it work?
The PACE CRISPR Detection Assay utilizes competitive allele-specific PCR with fluorescent reporting to accurately distinguish wild-type from edited sequences. Custom-designed primers precisely target your edit site, enabling sensitive detection of base substitutions, knockouts, indels, or SNPs created by CRISPR editing, even if edits are unknown.
Yes, PACE can identify mutations resulting from either NHEJ (random indels) or HDR (precise template-based edits).
PACE is faster, cheaper, and easier to scale for initial screens. Sequencing remains valuable for final validation, but PACE is ideal for early-stage or bulk population screening.
Each 400 µL aliquot is sufficient for at least 2,800 reactions at a standard 10 µL volume.
Yes. You can choose to receive the unvalidated assay for in-house use or send us your DNA samples for validation before shipment.
From PCR setup to results, the process typically takes 2–4 hours. Assay design and shipping timelines vary based on validation and synthesis steps.
Any DNA source—including crude lysates from cells, seeds, leaf tissue, or microbes—is compatible.
PACE detects a wide range of edits: single-nucleotide changes (SDN1), small and large insertions/deletions (SDN2/3), gene knockouts, and rearrangements. It can also monitor for off-target effects.
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John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
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