Custom assays to detect the presence of your target edit—whether introduced through directed editing or Non-Homologous End Joining (NHEJ)—designed using DNA sequences submitted by you, including the target site, spacer, and PAM. Assay information is submitted via the provided template and guidelines.
While high-throughput sequencing remains a common approach for screening, it is often time-consuming, costly, and data-intensive. PACE® (PCR Allele Competitive Extension) genotyping offers a rapid, scalable, and cost-effective alternative for primary screening of genome edits, widely applicable in both agricultural and life sciences research.
Confidentiality Assured: Assay primers are proprietary to each client. Each order includes 400 µL of ready-to-use 72x PACE assay, enough for at least 2,800 reactions at a 10 µL volume.
How does it work?
The PACE CRISPR Gene Edit Detection Assay uses competitive allele-specific PCR with fluorescent reporting to distinguish between wild-type and edited sequences. Custom-designed primers target the site of edit, allowing precise detection of base substitutions, knockouts, small indels, or SNPs introduced via CRISPR editing – whether the resultant edits are known or unknown.
Yes, PACE can identify mutations resulting from either NHEJ (random indels) or HDR (precise template-based edits).
PACE is faster, cheaper, and easier to scale for initial screens. Sequencing remains valuable for final validation, but PACE is ideal for early-stage or bulk population screening.
Each 400 µL aliquot is sufficient for at least 2,800 reactions at a standard 10 µL volume.
Yes. You can choose to receive the unvalidated assay for in-house use or send us your DNA samples for validation before shipment.
From PCR setup to results, the process typically takes 2–4 hours. Assay design and shipping timelines vary based on validation and synthesis steps.
Any DNA source—including crude lysates from cells, seeds, leaf tissue, or microbes—is compatible.
PACE detects a wide range of edits: single-nucleotide changes (SDN1), small and large insertions/deletions (SDN2/3), gene knockouts, and rearrangements. It can also monitor for off-target effects.
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