ProbeSure Genotyping Master Mix combines class-leading performance with the most competitive prices in the market. ProbeSure Genotyping Master Mix has provided users with accurate, robust, and consistent performance for many years. It has been carefully optimised to work over a wide range of reaction volumes and sample template qualities.
ProbeSure Genotyping Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.
Any dual-labelled probe with a fluorescent label at the 5′ end and a quencher at the 3′ end, such as TaqMan™, ZEN™ probes and BHQ® and BHQplus® probes, can all be used with ProbeSure Genotyping Master Mix.
Negative controls are very important to establish that the amplification being seen is real. Negative controls should always be used and should consist of the buffer the DNA samples are hydrated in.
Positive controls can be included to increase confidence that the correct genotypes are assigned to the samples, though this is not necessary in all cases. Positive controls should be samples of known genotype and are generally of more use when assessing a very low frequency variant.
No, ProbeSure Genotyping Master Mix can only be used with 5′ nuclease probe-based (e.g., TaqMan) assays only. Similarly, 5′ nuclease assays cannot be used with PACE Genotyping Master Mix.
We have encountered customers using master mixes at final concentrations below the intended 1x. This may work in some instances. Use of any of our master mixes below (or indeed above) 1x final concentration is not recommended as it could potentially lead to erroneous results. All our master mixes have been developed such that their components are optimal for their intended use at 1x concentration in the final reaction mix.
We manufacture PACE and ProbeSure Genotyping Master Mixes with different ROX levels. PACE Genotyping Master Mix is available as a high ROX (500 nM), low ROX (25 nM), standard ROX (150 nM) and no ROX version. ProbeSure Genotyping Master Mix is available as a high ROX (500 nM), low ROX (25 nM) and no ROX version.
If you are unsure about which ROX level is required for your qPCR machine or plate reader, please refer to the PACE ROX Instrument Compatibility List and ProbeSure ROX Instrument Compatibility Lists, or contact 3CR Bioscience technical support email@example.com and we will advise you.
No. However, 5’ nuclease assays are available from a range of manufacturers, such as IDT.
No. ProbeSure Genotyping Master Mix is not designed for use with intercalating dyes. We do not currently make a product specifically or this application, though it is something we’d consider if enough customers request it.