The Mag Blood Pro Genomic DNA Extraction Kit isolates high-quality DNA from human and animal blood samples. Designed for flexibility, it delivers consistent yields and purity, ensuring reliable performance in PCR, qPCR, sequencing, genotyping, and other downstream applications.
The Mag Blood Pro Kit is optimised for genomic DNA extraction from whole blood samples collected with common anticoagulants, including:
The kit uses silica-coated magnetic beads and buffer chemistry that selectively binds DNA from leukocytes, while effectively removing inhibitors such as haemoglobin and haeme-derived compounds. The purified DNA is high-quality, suitable for sensitive applications such as PCR, qPCR, SNP genotyping, sequencing, and biobanking.
Multiple freeze–thaw cycles of whole blood should be avoided, as they may fragment DNA and reduce yield. The protocol can be adapted to different sample inputs (e.g., mammalian blood, avian blood with nucleated red cells) and offers both manual protocols for small-scale use and automation-ready workflows for high-throughput extraction.
Kit components (7 reagents):
For cfDNA, plasma or serum samples are recommended. Whole blood is not suitable for cfDNA extraction, as nucleases from lysed red cells can degrade cfDNA.
No. The kit can be used with the MagC 9600 Automated Nucleic Acid Extraction System (or similar platforms) following the step-by-step Automated Extraction Protocol provided, or with the Manual Protocol for bench-scale applications.
Store in vacutainers containing anticoagulants (e.g., sodium citrate). For short-term storage, keep refrigerated at 2–6 °C for up to 35 days. For long-term storage, freeze at –80 °C. Avoid repeated freeze–thaw cycles.
Up to 200 μL of whole blood or 20 μL of poultry blood per well.
DNA binds selectively to magnetic beads under specific buffer conditions. A magnetic field is applied to separate and purify the DNA, a process known as solid-phase extraction.
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John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
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