Affordable, High-Performance Genotyping for Crop Research & Breeding

Unlock the Potential of Precision Agriculture with PACE® by 3CR

Crop breeding is at the heart of global food security. To meet the growing demands of climate change, disease and pest pressure and rising populations, breeders and researchers need reliable, scalable tools that enable marker-assisted selection and genomic innovation.

At 3CR, we deliver exactly that. Our patented PACE® (PCR Allelic Competitive Extension) technology provides a cost-effective, high-throughput SNP genotyping solution designed to accelerate crop improvement — without the overheads of expensive probe-based chemistries.

From large-scale breeding programmes to academic research projects, PACE® helps you bring more resilient, higher-yielding and better-quality crops to market faster.

Why Crop Scientists Choose 3CR

Our customers range from global seed companies to university breeding programmes, all benefiting from the flexibility and affordability of PACE®. Here’s why:

Cost-Effective Alternative

PACE® requires only unlabelled primers, eliminating the need for expensive labelled probes. The result? Lower assay costs without compromising on data quality.

Optimised for High-Throughput Workflows

Designed for 384- and 1536-well plate formats, PACE® makes it easy to process thousands of samples in parallel — perfect for large breeding populations and quick turnaround.

Free SNP Assay Design

We provide free custom SNP assay design for customers purchasing PACE® master mixes. Simply share your SNP of interest and we’ll supply primer sequences ready for ordering.

Multiplexing Up to 4 SNPs

PACE® Multiplex allows up to four SNPs to be genotyped in one reaction. That means reduced consumables, labour and DNA input, without additional optimisation.

Universal Across Crop Species

From cereals and legumes to oilseeds and vegetables, PACE® is species-neutral and works with DNA from virtually any plant.

MARKER-ASSISTED SELECTION

Applications in Crop Genotyping

Our customers apply PACE® across a wide range of breeding and research programmes, including:

Marker-Assisted Selection

Rapidly identify individuals carrying favourable alleles for traits such as drought tolerance, disease resistance or improved nutrient use efficiency.

Applications in Crop Genotyping

Our customers apply PACE® across a wide range of breeding and research programmes, including:

Genomic Selection

Accelerate breeding cycles by integrating SNP data into genomic prediction models, improving selection accuracy and shortening time to market.

Applications in Crop Genotyping

Our customers apply PACE® across a wide range of breeding and research programmes, including:

Trait Mapping & QTL Studies

PACE® provides robust SNP genotyping for mapping studies, enabling researchers to link genetic markers with complex agronomic traits.

Applications in Crop Genotyping

Our customers apply PACE® across a wide range of breeding and research programmes, including:

Germplasm Characterisation

Evaluate genetic diversity within breeding populations or landraces, supporting pre-breeding programmes and conservation of valuable genetic resources.

Applications in Crop Genotyping

Our customers apply PACE® across a wide range of breeding and research programmes, including:

Hybrid Purity & Identity Testing

Confirm hybrid seed purity or verify parental lines with fast, accurate SNP assays.

Crop-Specific Examples

Cereals (wheat, maize, rice, barley): SNP-based screening for yield, milling quality and disease resistance.
Oilseeds (canola, sunflower): Trait mapping for oil content, herbicide tolerance and stress resistance.
Legumes (soybean, pea, lentil): Selection for nitrogen-fixing efficiency and pest resistance.
Vegetables (tomato, cucumber, pepper): Genotyping for improved shelf-life, fruit quality and pathogen resistance.

Already Sure? Let’s UPGRADE Crop Genotyping TOGETHER!

PACE® adapts seamlessly to every crop, providing the flexibility breeders need across diverse species.

Order your PACE® Master Mix now and streamline your workflows with unmatched speed, accuracy, and cost-efficiency—trusted by 600+ labs worldwide.

Simplifying Genotyping Workflows

We understand that integrating new technologies can feel daunting. That’s why PACE® is designed for straightforward implementation in existing PCR workflows. Our mixes are supplied at 2x concentration, with or without ROX dye, and compatible with major qPCR instruments.

Not ready to run assays in-house? 3CR can deliver high-quality genotyping services using PACE® chemistry directly from our labs.

Get Started with PACE® for Crop Genotyping

Whether you’re breeding the next generation of staple crops or running research into plant adaptation and resilience, 3CR is here to support your goals.

PACE® Genotyping Master Mix

A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.

Volume

ROX Conc

Concentration

Clear

PACE® 2.0 Genotyping Master Mix

An enhanced PCR master mix for allele-specific assays. Improved signal to noise ratio and tight clustering. Developed specifically for genotyping direct from crude DNA samples.

Volume

ROX Conc

Concentration

Clear

PACE® Multiplex Master Mix

Our PACE Multiplex Master Mix enables simultaneous detection of up to four targets in a single reaction, saving time, reducing costs and consumables, and maximizing data generation.

Volume

Concentration

ROX Conc

Clear

PACE® OneStep RT-PCR Master Mix

Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.

Volume

ROX Conc

Concentration

Clear

PACE® Nano Genotyping Master Mix

Advanced performance at ultra-low reaction volumes, delivering consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.

Volume

ROX Conc

Concentration

Clear

CRISPR Gene Edit Detection Assay

PACE® CRISPR Detection Assay offers a cost-effective screening solution to detect CRISPR and other gene edits with speed and accuracy, making it ideal for high-throughput applications.

Volume

Service

Concentration

Clear

PACE® Genotyping Assay

Get custom PACE® genotyping assays precisely tailored to your target sequence. Our assays are fully compatible with all PACE® genotyping master mixes, ensuring reliable and accurate results for your genetic analysis.

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Service

Concentration

Clear

PACE® Master Mix Trial Kits

Everything you need to run a trial PACE Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.

Trial Kit Variant

Clear

MID- & HIGH-DENSITY GBTS MARKER PANELS FOR CROP GENOTYPING

Our targeted SNP marker panels deliver precise genotyping for crop breeding, traceability, and trait selection. Cost-effective, species-specific, and developed for high-performance applications in modern plant breeding and agricultural genomics.

what our clients say about us

“Thank you so much for being such a great company to work with. Your customer service has been wonderful. We are looking forward to developing more PACE markers linked to other disease resistant genes. Your PACE 2.0 Master Mix has worked beautifully. 3CR’s generosity to design PACE primers around SNPs is greatly appreciated. Thank you again.”

David C. Zlesak, Ph.D.

Professor of Horticulture, UWRF Plant and Earth Science Department

“I am very glad to see the level of support you provided on this troubleshooting, and I was happy to see such feedback from Marin who has years of experience with this assay – kudos to your team at 3CR!”

Director of R&D

Industry-leading Laboratory  Service Provider

“Over the course of several years, we have consistently experienced exceptional delivery times and communication from 3CR. The level of service they provide not only meets but greatly facilitates our global ambitions. Their commitment to excellence has proven to be a valuable asset, contributing significantly to the efficiency and success of our operations on a global scale.”

DKSH

“We have used PACE since modernizing our breeding program back in 2018. It is affordable, simple to use, and has never failed us! We constantly recommend it to others looking to expand their genotyping and marker-assisted selections programs!”

North Carolina State University

Peanut Breeding and Genetics program

“As a newly established company, finding cost-effective and approachable partners is essential. 3CR BIO has proven to be exactly that; offering competitive pricing without compromising on the quality of their products and services. Beyond their professionalism, I had the pleasure of meeting the team in person and can confidently say they are not only skilled but truly great people to work with. Whether you’re a large company or just starting out, 3CR BIO treats you with the same level of care and commitment’”

“The team at 3CR have been so thorough and helpful at troubleshooting even small genotyping irregularities.

PACE Nano is an exceptional product, massively improving data quality from difficult sample types or challenging assays.

We’ve enjoyed working with 3CR – they are efficient, responsive to our needs, and their products perform as promised.’”

“For a small horticultural seed company like ours that is in the process of modernizing its genotyping lab, we highly recommend working with 3CR’s professional team. We very much appreciate the accessibility and scientific expertise in the realm of molecular genetics in general and genotyping in particular to support us in reaching our breeding objectives as fast and efficiently as possible.”

“Working with 3CR has been remarkably smooth and supportive – their technology exceeded expectations, and their team truly understands the meaning of trust and long-term partnership.”

“3CR Biosciences delivers unmatched reliability and innovation in the field of agriculture research. They have strong technical and customer centric team. As their partners in India, iLife Biotech had an exciting journey to support scientists with fast, flexible and cost effective genotyping tools. The PACE assays has redesigned the genotyping workflows in the Indian Seed Companies.”

“We are very happy and super satisfied with the performance of PACE Genotyping. Our customers love PACE very much too! Also, the cost is very effective making PACE the ultimate genotyping solution especially for our agrigenomic customers in Indonesia.”

“The creativity in overcoming genotyping challenges has been instrumental to the success of the research and development function.”

“PACE Genotyping Mastermix has significantly improved the throughput and accuracy of our SNP genotyping projects. The mix is incredibly robust and easy to use, even with challenging DNA samples. We’ve reduced our reaction setup time and seen consistent results across hundreds of samples. It’s now our go-to solution for high-throughput genotyping.”

Mr.Ranjan Shaw

Biotechnology R&D, Namdhari Seed pvt ltd

“In our Biotechnology research lab, we’ve adopted PACE Genotyping Mastermix for SNP analysis across various plant and crops. The mix has proven to be highly reliable, delivering consistent and reproducible results even when working with low-concentration or partially degraded DNA samples. The simplicity of the protocol has also reduced hands-on time and minimized error rates during large-scale genotyping projects. It’s become a key component of our molecular biology workflow and has greatly supported our publication-quality data generation.”

Dr. Lakshmana Reddy Dc

Principal Scientist, ICAR-Indian Institute

“We are using PACE mastermix in our routine genotyping work. PACE master mix with features like work with crude DNA, tight and clear clusters on plot, cost effectiveness and versatile in nature makes excellent product to use.”

Mr.Vinay Jain

Scientist (Molecular Biology-R&D)

“We’re very satisfied with the quality and reliability of the genotyping solutions provided by 3CR, but above all, we truly value the close support and responsiveness the team has offered over the years.”

“My lab has relied on the 3CR PACE mastermix for years, and it has consistently delivered reliable, reproducible results. As a laboratory manager, I value how 3CR’s products and instrumentation help reduce per-sample costs, increase throughput, and accelerate assay development. Their exceptional support team and engineering innovations ensure low operating costs and minimal downtime, making them a vital partner in our workflow.”

“3CR Bioscience Limited provides us with DNA extracted from thousands of freeze-dried wheat seeds (we send in 96-deep well plates), offering a reliable and high-quality service.

DNA quality and quantity are perfectly suitable for further SNPs genotyping we perform by qPCR.

The support team is very efficient with easy communication. The delivery of the DNA samples is fast and safe, using robust packing.

Service highly recommended!”

Emilie JACOB (CAVEL)

End-to-end genotyping solutions FOR AQUACULTURE BREEDING

Unlock the full spectrum of genotyping possibilities with our comprehensive, modular range of instruments. Explore our end-to-end genotyping automation solutions and discover the perfect fit for your laboratory, ensuring efficiency and accuracy at every step.

AUTOMATED EXTRACTION

Automated Extraction

Fully automated, high-quality nucleic acid extraction & purification instrumentation for use with magnetic bead-based reagents. Suitable for genotyping, library sequencing, gene chip analysis & more.

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LIQUID HANDLING

LIQUID HANDLING

Automated liquid handling workstation for sample replication and reformatting, low volume assay dispensing, plate sealing and more. On-deck sonicating washing dramatically reduces tip consumption and cost.

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THERMAL CYCLING

THERMAL CYCLING

A highly efficient, high-throughput thermal cycling water bath instrument capable of running up to 25 PCR plates per run. Robust, streamlined basket design.

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DATA ANALYSIS

DATA ANALYSIS

A high-speed plate scanner for precise fluorescence signal detection and analysis. Full-field scanning technology for excellent sensitivity and resolution; automated loading, detection and unloading.

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About 3CR

3CR is dedicated to Making Science Affordable. Our PACE® technology powers research and breeding programmes worldwide, across crops, livestock, aquaculture, fruits and vegetables. By offering reliable, low-cost SNP genotyping, we help researchers and producers deliver innovations that matter.

PACE genotyping accuracy, independently verified

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YEARS OF COMBINED TECHNICAL EXPERIENCE

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customers worldwide

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assays designed to date

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seed producers worldwide trust PACE genotyping

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Take the Next Step! Precision breeding starts with precision tools.

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MEET OUR TEAM

Dr. John Holme Technical Director

Dr. Nisha JainOperations Director

Nazma Saffin General Manager

Jon Curtis Non-Executive Chair