Crop breeding is at the heart of global food security. To meet the growing demands of climate change, disease and pest pressure and rising populations, breeders and researchers need reliable, scalable tools that enable marker-assisted selection and genomic innovation.
At 3CR Bio, we deliver exactly that. Our patented PACE® (PCR Allelic Competitive Extension) technology provides a cost-effective, high-throughput SNP genotyping solution designed to accelerate crop improvement, without the overheads of expensive probe-based chemistries.
From large-scale breeding programmes to academic research projects, PACE® helps you bring more resilient, higher-yielding and better-quality crops to market faster.
Crop genotyping is the analysis of specific, known positions in a plant genome to identify which genetic variants an individual carries. Rather than sequencing the whole genome, it reads defined markers, usually single nucleotide polymorphisms (SNPs), which makes it fast and inexpensive enough to screen entire breeding populations. Breeders use the resulting marker data to select plants carrying desirable traits years before those traits can be observed in the field.
Crop genotyping underpins marker-assisted selection, genomic selection, QTL mapping, germplasm characterisation and hybrid purity testing. All five applications run on the same PACE® chemistry and the same plate-based workflow.
Our customers range from global seed companies to university breeding programmes, all benefiting from the flexibility and affordability of PACE®. Here’s why:
We provide free custom SNP assay design for customers purchasing PACE® master mixes. Simply share your SNP of interest and we’ll supply primer sequences ready for ordering.
From cereals and legumes to oilseeds and vegetables, PACE® is species-neutral and works with DNA from virtually any plant.
MARKER-ASSISTED SELECTION
PACE® adapts seamlessly to every crop, providing the flexibility breeders need across diverse species.
Order your PACE® Master Mix now and streamline your workflows with unmatched speed, accuracy, and cost-efficiency—trusted by 600+ labs worldwide.
We understand that integrating new technologies can feel daunting. That’s why PACE® is designed for straightforward implementation in existing PCR workflows. Our mixes are supplied at 2x concentration, with or without ROX dye, and compatible with major qPCR instruments.
Not ready to run assays in-house? 3CR can deliver high-quality genotyping services using PACE® chemistry directly from our labs.
Whether you’re breeding the next generation of staple crops or running research into plant adaptation and resilience, 3CR is here to support your goals.
Next-generation allele-specific PCR mix with enhanced signal clarity and robust performance, even from crude DNA samples.
Detect up to four targets in one PCR reaction. Save time, reduce consumables, and maximise throughput with effortless multiplexing.
Universal PCR mix for allele-specific assays. Delivers precise fluorescence, high performance, and consistent genotyping across reaction volumes.
Genotype directly from RNA. Combines reverse transcription and PCR amplification in a single, streamlined reaction.
High-performance, ultra-low-volume PCR mix for cost-effective, high-throughput genotyping in automated or miniaturised workflows.
Fast, affordable PACE®-based screening to detect CRISPR and gene edits with high accuracy in high-throughput applications.
Custom-designed PACE® genotyping assays for your specific target sequence, including primer and probe design for accurate allele discrimination and full compatibility with PACE master mixes.
All-in-one kit to trial PACE® genotyping. Includes assays, master mix, control DNA, and a detailed setup guide.
Our targeted SNP marker panels deliver precise genotyping for crop breeding, traceability, and trait selection. Cost-effective, species-specific, and developed for high-performance applications in modern plant breeding and agricultural genomics.
Unlock the full spectrum of genotyping possibilities with our comprehensive, modular range of instruments. Explore our end-to-end genotyping automation solutions and discover the perfect fit for your laboratory, ensuring efficiency and accuracy at every step.
3CR is dedicated to Making Science Affordable. Our PACE® technology powers research and breeding programmes worldwide, across crops, livestock, aquaculture, fruits and vegetables. By offering reliable, low-cost SNP genotyping, we help researchers and producers deliver innovations that matter.
Crop genotyping is used for marker-assisted selection, genomic selection, QTL and trait mapping, germplasm characterisation and hybrid purity testing. In each case it identifies which genetic variants a plant carries so that breeders can select the right individuals before field performance is visible.
Marker-assisted selection uses a small number of markers with a known link to a specific trait, such as a disease resistance gene. Genomic selection uses genome-wide marker data in a statistical model to predict the overall breeding value of an individual. MAS suits traits controlled by few genes, genomic selection suits complex traits such as yield.
PACE® is species-neutral and works with DNA from virtually any plant. Customers apply it across cereals such as wheat, maize, rice and barley, oilseeds including canola and sunflower, legumes such as soybean, pea and lentil, and vegetables including tomato, cucumber and pepper.
PACE® is designed for 384- and 1536-well plate formats, which allows thousands of samples to be processed in parallel. PACE® Multiplex genotypes up to four SNPs in a single reaction, reducing consumables, labour and DNA input further.
Partner with 3CR to drive innovation in crop genetics and secure a more productive future.
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John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
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