Universal PCR mix for allele-specific assays. Delivers precise fluorescence, high performance, and consistent genotyping across reaction volumes.
PACE® Master Mix delivers unmatched accuracy and performance for SNP genotyping. Designed for fluorescence-based, high throughput workflows with free assay design.
Universal PCR mix for allele-specific assays. Delivers precise fluorescence, high performance, and consistent genotyping across reaction volumes.
More than 600+ Universities and Businesses trust in us.
Tired of PCR reagents with high costs and slow delivery? PACE® Master Mix is built for labs that demand speed, accuracy, and high-throughput performance – engineered to meet the demands of fluorescence-based, high-throughput SNP genotyping.
Switching to PACE® Master Mix can save you up to 50% compared to other SNP genotyping reagents. Lower your cost per data point while maintaining the precision and throughput your work demands.
Whether you’re working with low-concentration or compromised DNA, PACE® delivers consistently high performance — giving you confidence in every result.
PACE® Master Mix integrates effortlessly with your existing workflow and assays. It’s fully compatible with all thermocyclers, fluorescence readers, and liquid handling platforms — and tailored for miniaturized reactions in 384-well formats. No retraining. No retooling. Just plug in, pipette, and go.
PACE® Master Mix has powered over 12 million SNP genotyping reactions across research institutions, biotech companies, and service labs.
OUR PACE® MASTER MIX RANGE OFFERS NEXT-LEVEL BENEFITS AND
APPLICATIONS
Our patented genotyping chemistry, PACE®, is the latest in allele-specific chemistry. It provides consistent performance making it ideal for high throughput genotyping applications. PACE® genotyping master mixes are designed for use with PACE® Genotyping Assays and are also compatible with pre-existing KASP™ and Amplifluor® assays.
Unlike probe-based products such as TaqMan™, PACE® assays only require un-labelled primer oligos to make up the PACE® genotyping reaction, in combination with PACE Genotyping Master Mix. Watch our quick video explaining how PACE® genotyping works.
All our master mixes are supplied at 2x concentration for convenience and contain ROX
normalising dye at either high (500 nM working concentration), low (25 nM working concentration), standard ROX level (150 nM working concentration) or without ROX.
Boost Your Genotyping Workflows with PACE® Multiplex!
Looking to double your data output while cutting down on DNA, consumables, and
labour?
PACE® Multiplex Master Mix makes it possible—no optimisation required!
| PACE | COMPETITOR | |
|---|---|---|
| Patented chemistry | ✓ | ✓ |
| SNP and Indel Detection | ✓ | ✓ |
| High Performance on Crude DNA Samples | ✓ | ✘ |
| Multiplex Genotyping | ✓ | ✘ |
| Real-Time Detection | ✓ | ✘ |
| One-step RNA Genotyping | ✓ | ✘ |
| Free & Confidential Assay Design Service | ✓ | ✘ |
| Assay Sequences Provided | ✓ | ✘ |
| Cost | $ | $$ |
Legal Information
KASP™ is a trademark of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
TaqMan™ is trademark of Roche Molecular Systems, Inc.
From crude DNA to multiplex SNP detection — our PACE® Master Mix range is engineered to match your exact application. Explore our proven formulations trusted by 600+ labs worldwide.
Universal PCR mix for allele-specific assays. Delivers precise fluorescence, high performance, and consistent genotyping across reaction volumes.
Next-generation allele-specific PCR mix with enhanced signal clarity and robust performance, even from crude DNA samples.
Detect up to four targets in one PCR reaction. Save time, reduce consumables, and maximise throughput with effortless multiplexing.
Genotype directly from RNA. Combines reverse transcription and PCR amplification in a single, streamlined reaction.
High-performance, ultra-low-volume PCR mix for cost-effective, high-throughput genotyping in automated or miniaturised workflows.
Custom PACE® assays designed for your target sequence. Ensure reliable, accurate allele detection with full PACE® compatibility.
Fast, affordable PACE®-based screening to detect CRISPR and gene edits with high accuracy in high-throughput applications.
All-in-one kit to trial PACE® genotyping. Includes assays, master mix, control DNA, and a detailed setup guide.
Unsure which mix to choose? Email us for help choosing the right mix for your application
Get started with PACE® now — optimized for speed, savings, and seamless integration with your current workflow. No commitments. Just results.
PACE Master Mix is compatibile with most qPCR machines, thermal cyclers and fluorescence plate readers. You can check compatibility with our PACE Instrument Compatibility Guide here.
Yes, PACE Master Mix works well with low purity, low concentration DNA samples. Purification of DNA will generally lead to better genotyping data quality than using a crude extract, but PACE Genotyping Master Mix works well with most crude DNA extraction methods. For some crops such as palm oil, where inhibitor concentration is high in crude extracts, we recommend the use of PACE 2.0 Genotyping Master Mix, which contains inhibitor-resistant components.
Unlike 5’ nuclease based probe-based products such as TaqMan™, PACE genotyping reactions only require un-labelled primer oligos to make up the PACE genotyping reaction, in combination with PACE Genotyping Master Mix, making them much more cost-effective, and quicker and easier to create new marker assays.
The PACE Genotyping Trial Kits contain everything you need to run a trial PACE® allele-specific PCR Genotyping Reaction on your existing lab equipment. It comes with a set of validated DNA samples, SNP assays, and PACE Master Mix.
Available for PACE Genotyping Master Mix, PACE 2.0 Genotyping Master Mix, PACE Multiplex Master Mix, and PACE Nano Genotyping Master Mix.
Whether you’re a seasoned scientist looking to adopt a new genotyping method or a novice researcher venturing into the realm of genomics, the PACE PCR Genotyping Trial Kit offers an ideal starting point.
At 3CR Bioscience, we are dedicated to your success and committed to supporting you throughout your genotyping journey. Our team of experts is always available to assist you at any stage, from assay design and set up to running the experiments and data analysis. We have a responsive and highly experienced support team here at 3CR bio which you can access at any time during set up by emailing support@3crbio.com.
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John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
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