Genotyping Technology in Action

3CR Bioscience is dedicated to developing cutting-edge innovation and exceptional products for the life sciences, with an emphasis on PCR genotyping technology and molecular markers for plant, animal, human and microbial genetic research.

From the developers of KASP™ and PACE® technology, PACE Genotyping Master Mix continues our pedigree of innovation and exceptional performance, characterised by extremely low non-specific amplification, and universal compatibility with allele-specific PCR assays including PACE, KASP, and Amplifluor®. Full functionality at all reaction volumes and plate-well densities (96-, 384-, 1536-well and array tape), our reagents undergo rigorous quality control and tracking procedures. All batches of mix are performance tested and certified, with Certificate of Analysis available for every batch, available to download from our website.

ProbeSure™ Genotyping Master Mix combines class-leading performance with the most competitive prices in the market. Our ProbeSure Genotyping Master Mix is designed for use with 5’ nuclease assays and is suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes. ProbeSure Genotyping Master Mix has been carefully optimised to work over a wide dynamic range of reaction volumes and sample template qualities. 

In an era of rapid breakthroughs in genomics, our hub of scientific excellence with 100+ years of combined experience in developing high-performance molecular biology tools is perfectly positioned to help you advance rapidly towards your goals.

We have successfully converted thousands of validated SNP targets into breeder-friendly markers for marker-assisted-selection studies across the globe. Let us help you avoid common pitfalls of marker assay design with our free allele-specific PACE assay design service and full-or partial assay validation options. All that is needed is short sequence information surrounding the variant of interest (e.g., SNP or Indel) and a qPCR machine or fluorescent plate reader.

Ready to run? For PACE®, KASP™ and any other similar PCR markers, our patented PACE Genotyping Master Mix gives exceptional performance, characterised by its extremely low non-specific amplification, and universal compatibility with allele-specific PCR assays including PACE, KASP, and Amplifluor®. 3CR Bioscience’s PACE Genotyping Master Mix provides unparalleled accuracy, affordability and reliability at any reaction volume, making it scalable and simple to integrate efficiently into any sized study, small or large.

DNA purification steps can be costly and time-consuming, especially when processing thousands of samples within tight deadlines, as many of our customers do. With the latest PACE® 2.0 formulation, you can now obtain superior data from your samples without the need for extensive purification.

PACE 2.0 Genotyping Master Mix ensures an unrivalled signal-to-noise ratio and produces tight clusters even when working with high-throughput, crude DNA preps, resulting in consistently exceptional performance. Efficiently streamline your workflow and reduce costs without compromising the quality of your results.

PACE 2.0 Genotyping Master Mix excels in handling crude DNA samples, making it the ideal solution for challenging starting material. PACE 2.0 has been specially formulated to overcome the obstacles presented by common PCR inhibitor compounds, such as phenols and tannins found in plant material. Even notoriously tricky samples, like oil palm and conifers, can be reliably assayed using hot shot or other crude DNA prep methods and still deliver reliable and accurate data.

3CR Bioscience has developed precision solutions for rapid pathogen detection, applicable right across agricultural, veterinary, and medical disciplines.

We have taken our patented PACE® chemistry one step further and created PACE OneStep RT-PCR, a fluorescent PCR detection system that can be customised to detect any DNA or RNA-based pathogens. Recent examples include the rapid, large-scale detection of agricultural disease outbreaks and the monitoring of circulating SARS-COV-2 variants within a population.

PACE OneStep RT-PCR has been adopted as a novel, rapid screen for the single-stranded RNA Cotton leafroll dwarf virus (CLRDV) in cultivated cotton by a group at Texas A&M University (read publication). CLRDV is a single-stranded RNA virus that poses a major global threat to cotton production, and current detection methods are expensive and time-consuming. PACE OneStep RT-PCR Master Mix (RT relating to the addition of reverse transcriptase) combines RNA transcription and cDNA amplification into a single reaction, using sequence-specific fluorescent primers to differentiate between infected and uninfected samples. PACE OneStep RT-PCR Master Mix can be applied to screens for DNA or RNA viruses in other crops and even animal species by targeting conserved virus- and host-specific genes.

Beyond pathogen detection, PACE OneStep RT-PCR genotyping has been adapted as a highly sensitive and accurate tool to rapidly identify variants within a circulating SARS-COV-2 population.

During the recent SARS-CoV-2 outbreaks, tracking genetic variations from positive samples has yielded crucial information about the number of variants circulating in each new outbreak and the possible lines of transmission. However, conventional screening by sequencing high numbers of positive SARS-CoV-2 samples is costly for population-scale test and trace operations.

A PACE OneStep RT-PCR Master Mix SNP identification pipeline, developed by School of Biological Sciences at the University of Bristol with 3CR Bioscience, offers a high-throughput, accurate, and affordable means to screen positive SARS-CoV-2 samples and monitor SNP variants in an outbreak. The publicly available pipeline is periodically updated as viral genotypes arise or disappear from circulation. The flexibility and versatility of PACE OneStep RT-PCR Master Mix means existing SNP panels can be quickly and inexpensively modified to keep pace with changes in strain emergence, providing valuable insight for epidemiological studies.

PACE® Multiplex Master Mix is a powerful and versatile extension of our PACE 2.0 Genotyping Master Mix, allowing for the simultaneous detection of multiple targets within a single PCR reaction.

Genotyping with PACE Multiplex Master Mix combines the principles of PCR amplification with the specificity of allele-specific extension and fluorescent labelling. This breakthrough evolution of our flagship technology enables researchers to assess multiple genotypes in a single reaction, reducing time, cost, and the need for additional samples and consumables. PACE Multiplex Master Mix genotyping has widespread applications in fields such as marker-assisted selection, transgenic plant development, seed testing, and disease diagnostics. Recent customer applications include quad-allelic SNP detection in cancer diagnostics, adventitious presence sequence detection in seed quality control testing, and pathogen detection on commercially grown crops. The flexibility and scalability of PACE Multiplex Master Mix make it an indispensable genotyping tool for higher throughput requirements.

At 3CR Bioscience, we are dedicated to your success and committed to supporting you throughout your genotyping journey. Our team of experts is always available to assist you at any stage, from assay design and set up to running the experiments and data analysis.

Assay design: Our team has been designing PCR genotyping technology assays for decades, and we are happy to share our expertise with you to achieve assays with optimal performance and accuracy. We offer a free PACE® assay design service for customers to save time and help reduce the cost and turnaround time of your genotyping. From assay validation to protocol implementation, we provide comprehensive technical support, addressing any challenges that arise. There isn’t much we can’t solve, and our dedication and customer communication are second to none.

Technical resources: Count on us for comprehensive resources and guidance during assay running and data analysis. Troubleshooting is part and parcel of what we do in our own labs and for our customers – over the years, we have seen it all! Our aim is to empower you with the skills and knowledge that will maximise the rapid production of top-quality data routinely in your facility, regardless of its size or your level of experience.