ProbeSure OneStep RT-PCR Master Mix is designed for highly specific and sensitive one-step reverse transcriptase (RT) fluorescently-reporting PCR using RNA template.
The master mix includes optimised components which allow reverse transcription and subsequent PCR amplification to take place in the same reaction well.
ProbeSure OneStep RT-PCR Master Mix may be used in endpoint or real-time modes.
The ProbeSure OneStep RT-PCR Master Mix reaction is comprised of two parts:
- Assay mix comprising forward and reverse primers, and fluorogenic hydrolysis probes.
- Universal ProbeSure OneStep RT-PCR Master Mix containing all the components required for reverse transcription and subsequent PCR.
When combined with template RNA, these components create a ProbeSure OneStep RT-PCR.
ProbeSure OneStep RT-PCR Master Mix is designed for use in PCR genotyping applications with hydrolysis probe-based assays. It can be used with a variety of fluorogenic probe types including TaqMan®, ZEN™ probes and BHQ® / BHQplus® probes.
