PCR GENOTYPING Products

Exceptional quality genotyping reagents, assays, and consumables. Generate consistent, accurate data at any volume, with scalable and cost-effective solutions.

pace®

PCR Allelic Competative Extension

PACE® is our patented PCR genotyping chemistry designed for allele-specific PCR genotyping. It offers excellent performance and value and is compatible with PACE®, KASP™, and Amplifluor® assays; it also supports Multiplex PCR genotyping.

Unlike probe-based systems like TaqMan™, PACE combines unlabelled assay primers with a universal fluorescent reporting cassette in the PACE Genotyping Master to streamline reactions and reduce costs. PACE master mixes are supplied at 2x concentration and available with High, Low, Standard, or No ROX dye options. 

Free PACE Assay Design
PACE Master Mix customers can use our complimentary PACE Assay design service to create custom allele-specific assays for use with all our PACE Genotyping Master Mixes. We also offer full or partial assay validation options. PACE reagents deliver exceptional performance while reducing genotyping expenses. With our free assay-design service and dedicated expert support, making the switch to PACE is easy and risk-free.

Pace® Assays

Discover PACE Assays for a range of applications including genotyping and CRISPR gene edit detection. PACE is 3CR Bio’s patented PCR genotyping chemistry designed for allele-specific PCR genotyping. It offers excellent performance and value. Our in-house scientific team design assays to your target sequences and then provides you with the optimised primer designs and sequences for use with our PACE Genotyping Master Mix range.

Unlike probe-based systems like TaqMan, PACE Assays comprise simple unlabelled assay primers, using the universal fluorescent reporting cassette in the PACE Genotyping Master to for fluorescence signal generation and detection, simplifying assay development and reducing costs.

PACE assays are easily multiplexed for multi-target detection of up to four targets per reaction with no additional optimisation required. Our scientific team has decades of experience designing these assays for a wide range of applications.

PROBESURE™

ProbeSure™ Genotyping Master Mixes are designed for use with 5’ nuclease assays and are suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes. ProbeSure Genotyping Master Mixes are supplied at 2x concentration for convenience and with a range of ROX normalising dye levels.

ProbeSure Genotyping Master Mix has been rigorously tested and optimised to work over a wide dynamic range of reaction volumes and sample template qualities. ProbeSure Genotyping Master Mix combines class-leading performance with the most competitive prices in the market.

Consumables

A selection of high-quality consumables and tools compatible with 3CR Bio’s range of high-throughput extraction and genotyping instruments including standard and slimline 384-well plates; 3D and 2D barcoded racked tubes with accompanying capper/decapper tool and whole plate barcode scanner.

We also supply leaf sampling tools and leaf sample collection kits to complement our genotyping lab services.

High-speed whole rack barcode scanner with HD imaging for accurate decoding and automation.

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Disposable polypropylene tip combs for use with our H9600 and H384 DNA extraction instruments.

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Discover our high-quality 96 deep well plates with a 2 ml capacity, designed specifically for extraction robots and automated workflows. These plates ensure reliable sample storage, chemical resistance, and consistent results in every application. Ideal for laboratories handling high-throughput processes, our 96 deep well plates (2 ml) are compatible with most extraction robots.

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DNA Extraction

Our MagPro range of magnetic bead-based extraction reagents are suitable for high quality manual extractions from leaf, seed, tissue and blood samples.

Our MagPro reagents are also full compatible with our GeneExtract range of automated extraction instruments – obtain high-quality, consistent DNA samples from up to 384 samples per run with full, walk-away automation.

For Research and Development purposes only. Not for diagnostic use.

Legal Information
TaqMan™ is a registered trademark of Roche Molecular Systems, Inc.
Zen™ Probe is a trademark of Integrated DNA Technologies, Inc.
KASP™, BHQ™ Probes, and BHQplus™ Probes are trademarks of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA

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MEET OUR TEAM

Steve Asquith Managing Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John Holme Technical Director
John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base. During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Dr. Nisha JainOperations Director
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP. Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nazma Saffin General Manager
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Jon Curtis Non-Executive Chair
After 8 years in The Royal Air Force, Jon moved to the Imperial Cancer Research Fund where he pioneered the use of ultra high-throughput genomic automation, capable of 46,000 PCRs per hour. In the 1990’s Jon joined GlaxoSmithKline, implementing a high-throughput genomics platform into their drug discovery pipeline. Whilst there he also developed acoustic mixing into compound management, becoming the gold standard across pharma. Jon developed the world’s first commercially viable 1536-well PCR plates, automated thermal & laser plate-sealer, plus automated liquid-handling & tip washing tools to reduce waste and costs. In 2002 Jon co-founded KBioscience with Phil Robinson, utilising ultra high-throughput PCR instrumentation & a suite of automation tools to create the company’s SNPline robotic platform, with a capacity of 250,000 PCRs/day. The business was underpinned by their ground-breaking patented genotyping chemistry, KASP™, which has over 10,000 scientific papers to date. In November 2022 Jon joined 3CR Bioscience acting as an advisor bringing his commercial and scientific experience to the company.