Multiplex Allele-Specific PCR Has Arrived

In the realm of genetic research and molecular biology, accuracy and efficiency are paramount. Scientists are constantly seeking innovative solutions to streamline processes while maximizing data yield. Enter PACE® Multiplex Master Mix – a game-changer in PCR genotyping, offering simultaneous detection of up to four targets in a single reaction. This advanced version of the flagship PACE® technology combines PCR amplification with allele-specific PCR extension and four-fluor fluorescent labelling, elevating allele-specific PCR and SNP genotyping to new heights.

Powering Discoveries Across Diverse Fields

PACE Multiplex Master Mix is an advanced and versatile extension of 3CR Bio’s PACE 2.0 Genotyping Master Mix. PACE Multiplex Master Mix isn’t just a tool; it’s a catalyst for progress across diverse domains such as disease research, marker-assisted selection, transgenic plant development, seed testing, and beyond. Its adaptability and scalability make it an invaluable asset for high-throughput needs, revolutionising the way researchers approach genotyping.

Bringing Innovation to Your Lab Bench

In the ever-evolving landscape of genetic research, staying ahead means embracing innovation. Imagine being able to detect quad-allelic SNP variations in cancer diagnostic research, identify adventitious presence sequences in seed quality control testing, or pinpoint pathogens in commercial crops, all in a single reaction. With PACE Multiplex Master Mix, you can save time, cut costs, and maximise data yield, all while pushing the boundaries of what’s possible in genomic research and selection.

Seamless Integration with Your Lab Equipment

One of the key features of PACE Multiplex Master Mix is its compatibility with a wide range of laboratory equipment. PACE Multiplex Master Mix can be used on any qPCR machine capable of detecting FAM, HEX, ATTO 590, and ATTO 647N fluorophores, a feature shared by most commercial qPCR machines used today. Alternatively, plate readers equipped with monochromators that can utilise at least five channels with a 10 nm bandwidth are also fully compatible with PACE Multiplex Master Mix. An additional ATTO 680 passive reference dye has been added toPACE Multiplex Master Mix for signal normalisation, to eliminate the effect of well to-well liquid volume differences when the cluster plot data is reviewed.

Sustainability: Halve Your Plastic and Reagent Consumption

The ability to detect two SNPs in a single reaction means users not only halve the time required to generate target data, but significantly reduce the use of all plastics, reagents and other consumables required – saving money and crucially reducing the environmental impact of scientific studies.

Multiplex SNP Genotyping vs Singleplex – The Same High- Level Performance at Double the Rate

Users can benefit from the same high levels of accuracy and precision they have come to expect from our enhanced PCR Master Mix for allele-specific PCR PACE 2.0 Genotyping Master Mix. Multiplex Master Mix and PACE 2.0 Genotyping Master Mix perform comparably across a range of PACE Genotyping Assays.

The Versatility You Need: Storage Made Simple

PACE Multiplex Master Mix offers flexibility in storage, ensuring convenience without compromising performance. While it can be stored at 4°C for up to two weeks, for longer-term storage, it’s recommended to store the master mix at -20°C in smaller aliquots to minimize freeze/thaw cycles. This versatility further enhances its usability in various laboratory settings.

Better, Faster, Cheaper Solutions with 3CR Bio: Your Genotyping Your Way

For those seeking customised assay designs, 3CR Bioscience offers a complimentary PACE Genotyping Assay design service to all PACE Multiplex Master Mix customers, ensuring that everyone can access tailored, affordable solutions to meet their specific experimental needs. We also offer full or partial assay validation carried out onsite by our expert team (additional charges apply). Whether validated or not, we believe our assay design service provides significant time and cost savings and adds significant value to your projects.

Conclusion: Transform Your Genotyping Workflow Today

In conclusion, PACE® Multiplex Master Mix represents a paradigm shift in PCR genotyping, offering researchers and breeders a powerful tool to expedite their studies while maximizing data yield, budget, and improving sustainability. Its ability to simultaneously detect multiple targets in a single reaction, coupled with its adaptability and compatibility, makes it an indispensable asset for laboratories engaged in genetic research and analysis across diverse disciplines. With PACE Multiplex Master Mix, users can save time, costs, and resources while unlocking new insights into genomics. Revolutionize your genotyping workflow today with PACE Multiplex Master Mix.

Multiplex Genotyping with PACE Multiplex Master Mix
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Steve Asquith Managing Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John Holme Technical Director
John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base. During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Dr. Nisha JainOperations Director
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP. Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nazma Saffin General Manager
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Jon Curtis Non-Executive Chair
After 8 years in The Royal Air Force, Jon moved to the Imperial Cancer Research Fund where he pioneered the use of ultra high-throughput genomic automation, capable of 46,000 PCRs per hour. In the 1990’s Jon joined GlaxoSmithKline, implementing a high-throughput genomics platform into their drug discovery pipeline. Whilst there he also developed acoustic mixing into compound management, becoming the gold standard across pharma. Jon developed the world’s first commercially viable 1536-well PCR plates, automated thermal & laser plate-sealer, plus automated liquid-handling & tip washing tools to reduce waste and costs. In 2002 Jon co-founded KBioscience with Phil Robinson, utilising ultra high-throughput PCR instrumentation & a suite of automation tools to create the company’s SNPline robotic platform, with a capacity of 250,000 PCRs/day. The business was underpinned by their ground-breaking patented genotyping chemistry, KASP™, which has over 10,000 scientific papers to date. In November 2022 Jon joined 3CR Bioscience acting as an advisor bringing his commercial and scientific experience to the company.