Genotyping with Pace®: Precision PCR Master Mix for high throughput labs.

PACE® Master Mix delivers unmatched accuracy and performance for SNP genotyping. Designed for fluorescence-based, high throughput workflows with free assay design.

3CR Bioscience PACE Genotyping Trial Kit

Everything you need to run a trial PACE Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.

Trusted by leading research labs and Service Providers

More than 600+ Universities and Businesses trust in us.

Proven at Scale — Trusted by High-Throughput Labs Worldwide

PACE® Master Mix has powered over 12 million SNP genotyping reactions across research institutions, biotech companies, and service labs. Get started with a free PACE® Master Mix Trial Kit — evaluate the PACE® Master Mix at no cost with no obligation to purchase.

THE PACE® MASTER MIX RANGE

OUR PACE MASTER MIX RANGE OFFERS NEXT-LEVEL BENEFITS AND
APPLICATIONS

Our patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It
provides consistent performance making it ideal for high-throughput genotyping
applications. PACE genotyping master mixes are designed for use with PACE Genotyping
Assays and are also compatible with pre-existing KASP™ and Amplifluor® assays.

Unlike probe-based products such as TaqMan™, PACE assays only require un-labelled
primer oligos to make up the PACE genotyping reaction, in combination with PACE
Genotyping Master Mix. Watch our quick video explaining how PACE genotyping works.

All our master mixes are supplied at 2x concentration for convenience and contain ROX
normalising dye at either high (500 nM working concentration), low (25 nM working
concentration), standard ROX level (150 nM working concentration) or without ROX.

Boost Your Genotyping Workflows with PACE® Multiplex!
Looking to double your data output while cutting down on DNA, consumables, and
labour?

PACE® Multiplex Master Mix makes it possible—no optimisation required!

  • Detect up to four targets in a single reaction
  • Combine existing assays seamlessly
  • Streamline workflows & maximize efficiency
PACE COMPETITOR
Patented chemistry
SNP and Indel Detection
High Performance on Crude DNA Samples
Multiplex Genotyping
Real-Time Detection
One-step RNA Genotyping
Free & Confidential Assay Design Service
Assay Sequences Provided
Cost $ $$

Legal Information
KASP™ is a trademark of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
TaqMan™ is trademark of Roche Molecular Systems, Inc.

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Let’s Get You Started RIGHT AWAY!

You know the value of reliable, high-throughput genotyping. Why wait?

Order your PACE® Master Mix now and streamline your workflows with unmatched speed, accuracy, and cost-efficiency—trusted by 600+ labs worldwide.

PACE Genotyping Master Mix Bottles for Allele specific PCR

THE PACE® MASTER MIX RANGE

Get started with a free PACE® Master Mix Trial Kit — optimized for speed, savings, and seamless integration with your current workflow. Evaluate the PACE® Master Mix at no cost with no obligation to purchase.

FREQUENTLY ASKED QUESTIONS

Explore our full range of PACE Genotyping Master Mixes

  • PACE Genotyping Master Mix: A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.
  • PACE 2.0 Genotyping Master Mix: An enhanced PCR master mix for allele-specific assays. Improved signal to noise ratio and tight clustering. Developed specifically for genotyping direct from crude DNA samples.
  • PACE Multiplex Master Mix: PACE Multiplex Master Mix for the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.
  • PACE OneStep RT-PCR Master Mix: Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.
  • PACE Nano Master MixAdvanced performance at ultra-low reaction volumes, delivering consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.

Purification of DNA will generally lead to better genotyping data quality than using a crude extract, but PACE Genotyping Master Mix works well with most crude DNA extraction methods. For some crops such as palm oil, where inhibitor concentration is high in crude extracts, we recommend the use of PACE 2.0 Genotyping Master Mix, which contains inhibitor-resistant components.

If you are using a qPCR instrument, many of the software packages that are supplied with the instrument can analyse PACE genotyping reaction data in real-time and endpoint modes.

For some qPCR instruments, and plate readers, where the supplied software cannot perform endpoint genotyping analysis directly, it is possible to use MS Excel or alternative software packages for the analysis.

PACE Multiplex users will require a plate reader capable of reading FAM, HEX, ATTO 590, ATTO 647N and reference dye ATTO 680 (wavelengths in the PACE Multiplex Master Mix User Guide). PACE Multiplex Master Mix is supplied at 2x concentration for convenience and with or without ATTO 680 reference dye at a range of levels to ensure compatibility with your qPCR machine or reader.

PACE Multiplex Master Mix users will require a qPCR instrument capable of reading FAM, HEX, ATTO 590, ATTO 647N and reference dye ATTO 680 (wavelengths in the PACE Multiplex Master Mix User Guide). PACE Multiplex Master Mix is supplied at 2x concentration for convenience and with or without ATTO 680 reference dye at a range of levels to ensure compatibility with your qPCR machine or reader.

OUR CLIENTS ARE HAPPY - ARE YOU?

Find the Right PACE® Mix for Your Workflow

From crude DNA to multiplex SNP detection — our PACE® Master Mix range is engineered to match your exact application. Explore our proven formulations trusted by 600+ labs worldwide.

Evaluate the PACE® Master Mix at no cost with no obligation to purchase.

3CR Bioscience PACE Genotyping Trial Kit

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Contact us

MEET OUR TEAM

Steve Asquith Managing Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John Holme Technical Director
John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base. During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Dr. Nisha JainOperations Director
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP. Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nazma Saffin General Manager
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Jon Curtis Non-Executive Chair
After 8 years in The Royal Air Force, Jon moved to the Imperial Cancer Research Fund where he pioneered the use of ultra high-throughput genomic automation, capable of 46,000 PCRs per hour. In the 1990’s Jon joined GlaxoSmithKline, implementing a high-throughput genomics platform into their drug discovery pipeline. Whilst there he also developed acoustic mixing into compound management, becoming the gold standard across pharma. Jon developed the world’s first commercially viable 1536-well PCR plates, automated thermal & laser plate-sealer, plus automated liquid-handling & tip washing tools to reduce waste and costs. In 2002 Jon co-founded KBioscience with Phil Robinson, utilising ultra high-throughput PCR instrumentation & a suite of automation tools to create the company’s SNPline robotic platform, with a capacity of 250,000 PCRs/day. The business was underpinned by their ground-breaking patented genotyping chemistry, KASP™, which has over 10,000 scientific papers to date. In November 2022 Jon joined 3CR Bioscience acting as an advisor bringing his commercial and scientific experience to the company.