Our custom CRISPR detection assays are designed to identify your specific genome edits whether introduced via directed CRISPR editing or Non-Homologous End Joining (NHEJ) using DNA sequences you provide, including the target site, spacer, and PAM. Simply submit your assay details using our provided template and guidelines.
Traditional high-throughput sequencing can be time-consuming and expensive. The PACE® CRISPR detec tion assay offers a rapid, scalable, and cost-effective solution for primary screening of gene edits, making it ideal for both agricultural and life sciences research.
Confidentiality Assured: Each client’s assay primers are proprietary and confidential. Every order includes 400 µL of ready-to-use 72x PACE assay, enough for at least 2,800 reactions at a 10 µL volume.
How does it work?
The PACE CRISPR Detection Assay utilizes competitive allele-specific PCR with fluorescent reporting to accurately distinguish wild-type from edited sequences. Custom-designed primers precisely target your edit site, enabling sensitive detection of base substitutions, knockouts, indels, or SNPs created by CRISPR editing, even if edits are unknown.
Yes, PACE can identify mutations resulting from either NHEJ (random indels) or HDR (precise template-based edits).
PACE is faster, cheaper, and easier to scale for initial screens. Sequencing remains valuable for final validation, but PACE is ideal for early-stage or bulk population screening.
Each 400 µL aliquot is sufficient for at least 2,800 reactions at a standard 10 µL volume.
Yes. You can choose to receive the unvalidated assay for in-house use or send us your DNA samples for validation before shipment.
From PCR setup to results, the process typically takes 2–4 hours. Assay design and shipping timelines vary based on validation and synthesis steps.
Any DNA source—including crude lysates from cells, seeds, leaf tissue, or microbes—is compatible.
PACE detects a wide range of edits: single-nucleotide changes (SDN1), small and large insertions/deletions (SDN2/3), gene knockouts, and rearrangements. It can also monitor for off-target effects.
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