PCR 2.0 Master Mix

An enhanced PCR 2.0 MasterMix for allele-specific assays. Improved signal to noise ratio and tight clustering. Developed specifically for genotyping direct from crude DNA samples.

PCR 2.0 Mastermix: Reliable Results from Crude DNA Samples

PACE PCR 2.0 MasterMix ensures an unrivalled signal-to-noise ratio and produces tight data clusters, even when working with high-throughput, crude DNA preps, resulting in consistently exceptional performance.

Cost-Effective – Reduce genotyping costs by up to 50%
High Performance – Reliable results across different reaction volumes and platforms
Seamless Compatibility – Works directly with KASP™ assays and instruments
Expert Support – Backed by 20+ years of genotyping expertise
Quality Assured – Rigorous quality control on every batch

Request a free PACE PCR 2.0 Master Mix trial today

Discover how PACE 2.0 PCR master mix can enhance your genotyping and save you money. Get in touch for a free trial today!

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Explore the benefits of PACE 2.0 PCR Master Mix:
Speed, Savings, and Simplicity

Our most advanced PCR Master Mix for Peak Performance

Optimised signal to noise ratio

PACE PCR 2.0 Master Mix ensures unrivalled performance with improved signal-to-noise ratio and tight data clusters.

Perfect for challenging samples

PACE PCR 2.0 MasterMix has been specially formulated to overcome common PCR inhibitor compounds, such as phenols and tannins. Even notoriously tricky samples like oil palm and conifers can still be assayed using hot shot or other crude DNA prep methods with consistent and accurate data.

Use with crude DNA

Developed specifically for genotyping direct from crude DNA samples. Efficiently streamline your workflow and reduce costs without compromising the quality of your results. PACE 2.0 Genotyping Master Mix is compatible with most crude DNA extraction methods.

Proven at Scale — Trusted by High-Throughput Labs Worldwide

PACE® PCR 2.0 Master Mix has powered over 1 million SNP genotyping reactions across research institutions, biotech companies, and diagnostic labs.  With call rates consistently above 99%, it delivers the performance high-throughput labs rely on — batch after batch.

25+ Years

of hands-on experience in SNP genotyping

5000+ Products

sold to labs across 35+ countries

600+ Clients

Academic & biotech labs trust PACE® in daily workflows

>99% + Call Rate

Average SNP call rate — even with low-quality DNA

PCR Master mix 2.0 – High-Performance Genotyping, Even from Crude DNA

PACE 2.0 is engineered for reliability under pressure. Whether you’re working with crude DNA extractions or challenging sample types like oil palm or conifers, this advanced master mix consistently delivers tight data clusters and clean signal-to-noise ratios—even in high-throughput settings.

Why Choose PACE® 2.0?

Exceptional Data Clarity
Produces high-quality genotyping results with strong, machine-readable fluorescent signals—even from hot shot or inhibitor-rich DNA preps.
Robust to PCR Inhibitors
Specially formulated to overcome phenols, tannins, and other common PCR inhibitors for reliable performance with difficult samples.
Universal Compatibility
Features a novel, universal fluorescent reporting cassette and includes ROX normalization dye—fully compatible with your qPCR platform.
Flexible Assay Options
Supports competitive allele-specific genotyping designs and is also compatible with KASP™ and Amplifluor® markers.

	PACE	COMPETITOR
Patented chemistry	✓	✓
SNP and Indel Detection	✓	✓
High Performance on Crude DNA Samples	✓	✘
Multiplex Genotyping	✓	✘
Real-Time Detection	✓	✘
One-step RNA Genotyping	✓	✘
Free & Confidential Assay Design Service	✓	✘
Assay Sequences Provided	✓	✘
Cost	$	$$

Legal Information
KASP™ is a trademark of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
TaqMan™ is trademark of Roche Molecular Systems, Inc.

what our clients say about us

“Thank you so much for being such a great company to work with. Your customer service has been wonderful. We are looking forward to developing more PACE markers linked to other disease resistant genes. Your PACE 2.0 Master Mix has worked beautifully. 3CR’s generosity to design PACE primers around SNPs is greatly appreciated. Thank you again.”

David C. Zlesak, Ph.D.

Professor of Horticulture, UWRF Plant and Earth Science Department

“I am very glad to see the level of support you provided on this troubleshooting, and I was happy to see such feedback from Marin who has years of experience with this assay – kudos to your team at 3CR!”

Director of R&D

Industry-leading Laboratory  Service Provider

“Over the course of several years, we have consistently experienced exceptional delivery times and communication from 3CR. The level of service they provide not only meets but greatly facilitates our global ambitions. Their commitment to excellence has proven to be a valuable asset, contributing significantly to the efficiency and success of our operations on a global scale.”

DKSH

“We have used PACE since modernizing our breeding program back in 2018. It is affordable, simple to use, and has never failed us! We constantly recommend it to others looking to expand their genotyping and marker-assisted selections programs!”

North Carolina State University

Peanut Breeding and Genetics program

“As a newly established company, finding cost-effective and approachable partners is essential. 3CR BIO has proven to be exactly that; offering competitive pricing without compromising on the quality of their products and services. Beyond their professionalism, I had the pleasure of meeting the team in person and can confidently say they are not only skilled but truly great people to work with. Whether you’re a large company or just starting out, 3CR BIO treats you with the same level of care and commitment’”

“The team at 3CR have been so thorough and helpful at troubleshooting even small genotyping irregularities.

PACE Nano is an exceptional product, massively improving data quality from difficult sample types or challenging assays.

We’ve enjoyed working with 3CR – they are efficient, responsive to our needs, and their products perform as promised.’”

“For a small horticultural seed company like ours that is in the process of modernizing its genotyping lab, we highly recommend working with 3CR’s professional team. We very much appreciate the accessibility and scientific expertise in the realm of molecular genetics in general and genotyping in particular to support us in reaching our breeding objectives as fast and efficiently as possible.”

“Working with 3CR has been remarkably smooth and supportive – their technology exceeded expectations, and their team truly understands the meaning of trust and long-term partnership.”

“3CR Biosciences delivers unmatched reliability and innovation in the field of agriculture research. They have strong technical and customer centric team. As their partners in India, iLife Biotech had an exciting journey to support scientists with fast, flexible and cost effective genotyping tools. The PACE assays has redesigned the genotyping workflows in the Indian Seed Companies.”

“We are very happy and super satisfied with the performance of PACE Genotyping. Our customers love PACE very much too! Also, the cost is very effective making PACE the ultimate genotyping solution especially for our agrigenomic customers in Indonesia.”

“The creativity in overcoming genotyping challenges has been instrumental to the success of the research and development function.”

“PACE Genotyping Mastermix has significantly improved the throughput and accuracy of our SNP genotyping projects. The mix is incredibly robust and easy to use, even with challenging DNA samples. We’ve reduced our reaction setup time and seen consistent results across hundreds of samples. It’s now our go-to solution for high-throughput genotyping.”

Mr.Ranjan Shaw

Biotechnology R&D, Namdhari Seed pvt ltd

“In our Biotechnology research lab, we’ve adopted PACE Genotyping Mastermix for SNP analysis across various plant and crops. The mix has proven to be highly reliable, delivering consistent and reproducible results even when working with low-concentration or partially degraded DNA samples. The simplicity of the protocol has also reduced hands-on time and minimized error rates during large-scale genotyping projects. It’s become a key component of our molecular biology workflow and has greatly supported our publication-quality data generation.”

Dr. Lakshmana Reddy Dc

Principal Scientist, ICAR-Indian Institute

“We are using PACE mastermix in our routine genotyping work. PACE master mix with features like work with crude DNA, tight and clear clusters on plot, cost effectiveness and versatile in nature makes excellent product to use.”

Mr.Vinay Jain

Scientist (Molecular Biology-R&D)

“We’re very satisfied with the quality and reliability of the genotyping solutions provided by 3CR, but above all, we truly value the close support and responsiveness the team has offered over the years.”

“My lab has relied on the 3CR PACE mastermix for years, and it has consistently delivered reliable, reproducible results. As a laboratory manager, I value how 3CR’s products and instrumentation help reduce per-sample costs, increase throughput, and accelerate assay development. Their exceptional support team and engineering innovations ensure low operating costs and minimal downtime, making them a vital partner in our workflow.”

“3CR Bioscience Limited provides us with DNA extracted from thousands of freeze-dried wheat seeds (we send in 96-deep well plates), offering a reliable and high-quality service.

DNA quality and quantity are perfectly suitable for further SNPs genotyping we perform by qPCR.

The support team is very efficient with easy communication. The delivery of the DNA samples is fast and safe, using robust packing.

Service highly recommended!”

Emilie JACOB (CAVEL)

pace® genotyping TRIAL KIT

PCR Allele Competitive Extension

PACE® (PCR Allele Competitive Extension) Genotyping Master Mix is a patented master mix designed for use with allele-specific assays (e.g., PACE®, KASP™, Amplifluor®, etc…). It requires the addition of non-labelled oligos, unlike expensive dual-labelled probe chemistries.

PACE® PCR 2.0 Master Mix is supplied at 2x concentration for convenience and is supplied with ROX normalising dye at either high level (500nM working concentration), low level (25nM working concentration), standard ROX (150nM working concentration) or can be supplied without ROX.

3CR Bioscience also provides a free assay design service for use with the Master Mix, with the intention that customers can save money by purchasing their own oligos.

PACE® Master Mix Trial Kits

All-in-one kit to trial PACE® genotyping. Includes assays, master mix, control DNA, and a detailed setup guide.

Trial Kit Variant

Clear

THE PACE® MASTER MIX RANGE

Get started with a free PACE® PCR 2.0 MasterMix — optimized for speed, savings, and seamless integration with your current workflow.

FREQUENTLY ASKED QUESTIONS

What is a PCR master mix and why is it used in high-throughput fluorescence-based PCR?

A PCR master mix is a ready-to-use solution that contains essential PCR components—DNA polymerase, dNTPs, buffer, MgCl₂, and sometimes ROX or other reference dyes. It simplifies experimental setup, reduces pipetting errors, and ensures consistency across reactions, which is critical in high-throughput workflows.

Can PACE Genotyping Master Mixes be used with other genotyping platforms like KASP™ or Amplifluor®?

Yes. PACE Genotyping Master Mixes are fully compatible with assays designed for KASP™ and Amplifluor® platforms, making them a flexible choice for labs with existing assay designs.

What instrumentation is required to use PACE Genotyping Master Mixes?

PACE reactions can be analyzed using standard qPCR machines or fluorescent plate readers. The use of a ROX reference dye version of the mix depends on your detection platform, and 3CR provides guidance on ROX compatibility for various instruments.

Is there a cost advantage to using PACE over probe-based chemistries?

Yes. PACE does not require costly labeled probes—only standard oligos and master mix—significantly reducing assay development and running costs.

How do I get started with a new SNP or Indel assay using PACE?

Simply submit your SNP/Indel and flanking sequence to 3CR Bioscience. Their free PACE Assay Design Service will provide optimized primer designs, streamlining assay development and reducing setup time.

Is technical support available if I encounter issues with my genotyping experiments?

Yes. 3CR Bioscience provides expert-level support from assay design through data analysis. Their team has decades of experience and offers troubleshooting, guidance, and best practices tailored to your setup.

How is product quality ensured at 3CR Bioscience?

Each batch of PACE Master Mix undergoes a stringent QC process, including performance comparisons against historical batches, ensuring consistent, reproducible results. All Certificates of Analysis are available to download from our website support section.

END-TO-END SOLUTIONS FROM 3CR BIO

We specialize in innovative, cost-effective, end-to-end solutions for high-throughput genotyping and automation, no matter the scale.

PACE® & ProbeSure PCR Genotyping Reagents:
Ideal for ≤200 SNPs (Marker validation & Screening).

Genotyping Lab Services:
Including DNA Extraction, Genotyping, and GBTS.

Instrumentation:
Complete high-throughput genotyping automation pipelines and individual instruments.

Consumables:
384-well PCR plates, tubes, and seals

still Have questions? don’t hesitate to Send us a message.

MEET OUR TEAM

Dr. John Holme Technical Director

Dr. Nisha JainOperations Director

Nazma Saffin General Manager

Jon Curtis Non-Executive Chair