PCR Automation System for Genotyping and Automated PCR Setup
The HyperGeno™ is 3CR Bioscience’s most advanced, high-throughput automation system, delivering a fully integrated, end-to-end genotyping workflow from sample preparation to data analysis. Designed for large-scale molecular breeding and genomics laboratories, it enables true walk-away operation with maximum accuracy, scalability, and efficiency.
Maximum Throughput & Reproducibility
Integrates four high-performance modules into a scalable platform that significantly increases throughput while ensuring exceptional consistency and reliable results.
End-to-End Automation
Fully automates DNA dispensing, drying, reagent handling, PCR amplification, fluorescence scanning, and data analysis in one seamless workflow – eliminating manual intervention and reducing human error.
Efficiency & Cost Reduction
Minimises hands-on time and reagent consumption, optimising laboratory resources and lowering operational costs without compromising performance.
The HyperGeno™ Systems integrate the following modules:
Application
Ideal for ultra-large-scale SNP and InDel marker detection, the HyperGeno™ is widely applicable to molecular marker-assisted breeding, functional gene detection, GMO identification, purity testing, variety identification, molecular marker development and screening, association analysis and validation, fine gene mapping, and genetic diversity analysis. It provides a powerful, reliable foundation for establishing modern, high-capacity molecular breeding systems.
HyperArrayer
High-Throughput Multifunctional Liquid Handling Platform
The HyperArrayer is a fully automated liquid handling platform designed for ultra-high-throughput genotyping workflows. It integrates DNA dispensing, DNA drying, reagent dispensing, ultrasonic tip cleaning, and automated heat sealing into a single, streamlined system.
Using nanolitre-precision dispensing technology, the HyperArrayer accurately delivers DNA samples and reagents into high-precision microplates before automatically sealing them. An optional sample-drying module further reduces reagent consumption while minimising PCR inhibition caused by sample contaminants. By fully automating plate preparation, the HyperArrayer dramatically reduces manual handling and ensures exceptional accuracy, consistency, and reproducibility.
The system is optimised for 384-well plate formats and enables true walk-away processing of up to 400 × 384-well plates within 8 hours when operating as part of the integrated HyperGeno™ workflow.
HyperArrayer
- Ultra-High Throughput – Prepares up to 153,600 reactions in 8 hours
- High Automation – Loadable and removable tiered sample plate stackers and stacked reaction plate stackers allow continuous preparation of up to 80 reaction plates per run
- Miniaturised PCR Volumes – Supports reaction volumes as low as 1.6 µL
- Automated Sample Drying – Reduces PCR inhibition and substantially lowers reagent usage
- Automated Heat Sealing – Waste-free, consistent, water-tight sealing across every plate
- Advanced Cleaning Performance – Automated tip cleaning combined with ultrasonic technology eliminates cross-contamination
Instrument Details
- Supported plate formats: Standard and slim 384-well microplates
- Dispensing range:
- Contact dispensing: 0.8–10 µL
- Non-contact dispensing: 0.8–3 µL
- Deck positions: Four, each with independent temperature control
- Drying oven capacity: 15 plates
HyperCycler
High-Throughput Water Bath PCR Thermal Cycler
The HyperCycler is an automated, ultra-high-throughput water bath PCR thermal cycler engineered for large-scale PCR detection laboratories. A single run can simultaneously amplify up to 40 × 384-well PCR plates, dramatically increasing laboratory throughput.
The system features a flexible water bath basket, allowing users to load variable plate numbers depending on experimental requirements. With precise temperature control, excellent thermal uniformity, and high energy efficiency, the HyperCycler is ideally suited for continuous, large-volume PCR amplification.
Hypercycler Key Features
- High-Throughput Performance – Processes up to 15,360 reactions per run using 384-well plates
- Flexible Precision Control – Three independent water baths support two-step PCR, three-step PCR, Touchdown PCR, and other protocols
- Excellent Temperature Uniformity – Integrated circulating pump ensures stable and uniform temperatures across all plates
- High Efficiency & Energy Saving – Water-based heating eliminates ramp times associated with metal heating blocks, significantly reducing PCR cycle duration and achieving up to 80% higher energy efficiency
- User-Friendly Interface – 15.6-inch high-resolution touchscreen for rapid protocol setup and operation
Instrument Details
- Throughput: 40 × 384-well PCR plates or 1536-well PCR plates per run
- Water baths: 3, each with 33 L capacity
HyperScanner
Automated High-Throughput Fluorescence Scanner
The HyperScanner 4200 is a high-performance fluorescence microplate scanning system designed for miniaturised PCR volume detection applications requiring high sensitivity and accuracy. The system automates plate loading, barcode reading, and fluorescence detection to ensure fast, reliable, and reproducible data acquisition.
The HyperScanner 4200A configuration includes an automated stacker system, enabling fully unattended operation with continuous processing of up to 80 PCR plates per run, making it ideal for ultra-high-throughput genotyping laboratories.
HyperScanner 4200 Key Features
- Ultra-Fast Scanning – Full-field scanning technology reduces scan time per plate to just seconds
- High Sensitivity – Accurate detection for reaction volumes down to 0.8 µL
- Multi-Channel Detection – Six detection channels for multiplexed endpoint genotyping
Instrument Details
- Application: Endpoint fluorescence detection
- Scanning speed: < 8 seconds per channel
- Detection speed: 20 seconds per plate
- Scanning method: Full-field CMOS scanning
- Detection channels: FAM, HEX, ROX, ATTO590, ATTO647, ATTO680, with custom channel options available
Hypercycler 4200A Key Features
- Unattended Walk-Away Operation – Continuous processing of up to 80 PCR plates
- Easy Operation – Four removable stackers, each holding 40 PCR plates
- Efficient Re-Scanning – Dedicated recovery station enables fast rescanning of plates with barcode errors
- Upgradeable Design – The HyperScanner 4200 can be upgraded on-site to the 4200A; stacker installation is configurable to suit laboratory layouts
HyperMaster
Data Management and Analysis Software
HyperMaster is an advanced data management and analysis software platform developed specifically for ultra-high-throughput genotyping laboratories. It supports collaboration for up to 20 simultaneous users, with flexible permission management and powerful data processing capabilities.
The software provides integrated management of samples, molecular markers, reagents, and experimental protocols, alongside rapid raw data acquisition and automated cluster analysis. Robust export functionality enables direct output to Excel and easy report generation, including cluster plots, CSV files, scatter plots, and SNPviewer structure data.
HyperMaster Key Features
- Multi-User Collaboration – Supports up to 20 concurrent users
- Permission Management – Customisable access levels for operational and data analysis tasks
- Rapid Experiment Setup – Batch creation of sample plates, reagent plates, and locus information
- Advanced Analysis Tools – Cross-plate analysis, automated clustering, adaptive axis scaling, genotype plot watermarking, adjustable icon sizes, multi-image comparison, and customisable analysis parameters
Support & Services
3CR Bioscience develops tailored, cost-efficient solutions based on individual project requirements. Our team supports planning and feasibility assessments to ensure scientific robustness and practical implementation.
Installation & Training
We provide comprehensive installation and training programmes, including on-site commissioning, theoretical instruction, hands-on training, and long-term technical support to ensure reliable operation and user confidence.
After-Sales Support
We provide comprehensive application guidance, including technical support for primer design, locus development, and application-specific workflows to ensure optimal performance and reliable results. Our service also includes complimentary system upgrades, offering regular software updates and support tailored to customised functional requirements. In addition, we deliver flexible on-site service, with both remote and field-based support arranged according to each customer’s specific needs.
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