The Mag Plant Pro Genomic DNA Extraction Kit isolates high-quality DNA from a wide range of plant tissues, delivering excellent yields and purity for reliable downstream applications. The extraction method is based on the selective binding of DNA molecules to magnetic beads under specific buffer conditions, followed by separation and purification using a magnetic field – a process known as solid phase extraction.
The Mag Plant Pro Kit includes six reagents designed for efficient, selective DNA extraction:
This kit is optimised for a wide range of plants, including those rich in secondary metabolites (polyphenols, polysaccharides, lipids). Protocol adjustments are provided in the User Guide to maximize yield and purity. For best results, use fresh or young tissue, finely homogenized with liquid nitrogen grinding or mechanical disruption before lysis.
Available in three kit sizes:
The Mag Plant Pro Kit is offered in Small, Medium, and Large versions, each using the same optimized protocol but differing in total DNA yield. As a general guide, the Small Kit yields about 0.5 µg, the Medium Kit yields 1–1.5 µg, and the Large Kit yields ≥2 µg of purified DNA (actual yields vary by seed type and quality). Choose the version that best matches your downstream application and desired DNA output.
Yes. Optimization may be needed for species with challenging chemistries (high secondary metabolites, polysaccharides, or tough cell walls). The User Guide provides specific recommendations, such as adding DTT or β-mercaptoethanol, using liquid nitrogen grinding, or adding carrier RNA.
Yes, with optimizations (e.g., extended lysis, carrier RNA, proteinase K, concentrated elution). Note that degraded DNA may limit downstream applications.
No. The kit can be used with the MagC 9600 Automated Nucleic Acid Extraction System (or similar platforms) following the step-by-step Automated Extraction Protocol provided, or with the Manual Protocol for bench-scale applications.
Freeze at –80 °C. Dried materials should be processed within 1 month.
Standard protocols yield 10–30 kb fragments. For long-read sequencing (≥50 kb), additional specialized methods are recommended.
Up to 100 mg fresh or 30 mg dried tissue.
DNA binds selectively to the surface of magnetic beads under specific buffer conditions. A magnetic field is then used to separate and purify the DNA (solid-phase extraction).
Typically 50 mg fresh or 20 mg dried leaf tissue. This can be adjusted depending on species.
RNA removal depends on tissue type. For complete removal, RNase A can be added (10 µL RNase A per 600 µL Buffer PLS).
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