PACE® Genotyping Assay

Custom PACE® assays designed for your target sequence. Ensure reliable, accurate allele detection with full PACE® compatibility.
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genotyping assay

About

About PACE® (PCR Allelic Competitive Extension) genotyping assays deliver rapid, reliable identification of DNA sequence variants, including SNPs and Indels using powerful, homogeneous PCR-based allele-specific chemistry. Trusted in research and commercial applications globally, PACE assays offer unmatched accuracy and ease of use for all your genotyping needs.

PACE® genotyping technology consists of two core components:

PACE® Genotyping Assay:

Each custom-designed assay includes two allele-specific forward primers, each tailored to a unique variant at the 3’ end and equipped with a distinct 5’ tail for fluorescent signal generation, plus a common reverse primer. This precise design ensures highly specific and sensitive variant detection, even in complex samples.

PACE® Genotyping Master Mix:

Our proprietary master mix contains everything else you need for robust PCR amplification and fluorescent detection. The universal fluorescent reporting system produces clear, machine-readable signals (FAM and HEX), allowing fast and accurate genotype calls in any compatible instrument.

 

When combined with your DNA samples, these components form a seamless PACE genotyping reaction, streamlining your workflow from sample to result.

Our expert team brings years of experience in genotyping assay design, ensuring every PACE® assay is optimized for your specific targets. Choose from Validated Assays, rigorously tested and optimized with your DNA for guaranteed performance, or Unvalidated Assays, designed in silico and delivered rapidly for immediate use.

Whether you need precision, scalability, or speed, PACE® genotyping assays deliver results you can trust.

 

REQUIRED COMPONENTS

  • qPCR machine or Thermocycler + Fluorescent plate reader
  • PCR plate or equivalent and appropriate optically clear seal
  • Template DNA
  • PCR-grade water
  • PACE Genotyping Master Mix or PACE 2.0 Genotyping Master Mix

 

 

STEPS TO YOUR PACE GENOTYPING ASSAY DESIGN

  1. Place your order on this page. Our support team will contact you by email.
  2. Fill out an Assay Design Template form with all the information we need to process your custom PACE Genotyping Assay order. We will email you a copy of the template when we first contact you, or your can download a copy here.
  3. Email your completed Assay Design Template to support@3crbio.com
  4. Using the information you provide us, we will create your PACE Genotyping Assay designs, order the oligos, and send you design sequences.
  5. Once we receive the oligos, we assemble the assay(s) and then ship an aliquot to you (unvalidated) or test on your DNA samples before shipping the aliquot to you (validated).

Documentation

Price List PACE Genotyping Assay PACE Assay design template

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Documentation
PACE User Manual
PACE Certificate

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MEET OUR TEAM

Steve AsquithManaging Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John HolmeTechnical Director

John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.

During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.

Dr. Nisha JainOperations Director

Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.

Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.

Nazma SaffinGeneral Manager
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Greig PollandAutomation and Support Manager

Greig is a hands-on automation specialist and team leader with a strong background in laboratory and industrial automation. He has spent over 25 years developing, installing, and supporting automated systems that transformed laboratory workflows. During this time, Greig worked closely with scientists and engineers to tailor automation solutions for genotyping and molecular biology, an experience that sparked his lasting passion for combining technology with practical science.

Since then, Greig has built on that foundation through leadership roles where he leads automation and support operations. He’s known for being approachable, commercially minded, and deeply committed to helping teams and customers get the best from their technology.

Whether managing a complex automation rollout or helping a customer troubleshoot in real time, Greig brings a thoughtful, collaborative approach that keeps people ,not just machines, at the centre of what he does.