UPGRADE YOUR GENOTYPING WITH PACE®

PACE® genotyping reagents offer a seamless transition for researchers using KASP™ assays, providing accurate SNP and InDel analysis at up to half the cost. Compatible with your existing marker assays and instruments, PACE reagents deliver exceptional performance while reducing genotyping expenses. With our free assay-design service and dedicated expert support, making the switch to PACE is easy and risk-free.

WHY CHOOSE PACE®?

  • Cost-Effective – Reduce genotyping costs by up to 50%
  • High Performance – Reliable results across different reaction volumes and platforms
  • Seamless Compatibility – Works directly with KASP™ assays and instruments
  • Expert Support – Backed by 20+ years of genotyping expertise
  • Quality Assured – Rigorous quality control on every batch

REQUEST A PACE TRIAL TODAY!

Discover how PACE and 3CR Bio can enhance your genotyping workflow. Get in touch for a free trial of PACE Genotyping Master Mix.

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SWITCHING TO PACE IS EASY.

Transitioning to PACE is seamless. Simply use your currant PCR SNP and InDel marker assays with PACE Genotyping Master Mix on your existing thermal cycling instrument. Click here to view our comprehensive PACE Genotyping Master Mix User Guide and start saving time and money.

THE PACE® MASTER MIX RANGE

OUR PACE MASTER MIX RANGE OFFERS NEXT-LEVEL BENEFITS AND APPLICATIONS

PACE Genotyping Master Mix
A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance.

PACE 2.0 Genotyping Master Mix
An enhanced PCR master mix for allele-specific assays. Improved signal to noise ratio and tight clustering. Developed specifically for genotyping direct from crude DNA samples.

PACE Multiplex Master Mix
For the simultaneous detection of up to four targets in a single reaction. Save time, cost, and consumables while maximising data generation.

PACE OneStep RT-PCR Master Mix
Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.

PACE Nano Genotyping Master Mix
Advanced performance at ultra-low reaction volumes. Consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.

PACE Genotyping Assays
Custom designed PACE Genotyping Assays, designed to your target sequence. Compatible with all PACE genotyping master mixes.

PACE COMPETITOR
Patented chemistry ✓ ✓
SNP and Indel Detection ✓ ✓
High Performance on Crude DNA Samples ✓ ✘
Multiplex Genotyping ✓ ✘
Real-Time Detection ✓ ✘
One-step RNA Genotyping ✓ ✘
Free & Confidential Assay Design Service ✓ ✘
Assay Sequences Provided ✓ ✘
Cost $ $$

Legal Information
KASP™ is a trademark of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
TaqMan™ is trademark of Roche Molecular Systems, Inc.

OUR CLIENTS ARE HAPPY - ARE YOU?

3CR Bioscience Logo

David C. Zlesak, Ph.D.
Professor of Horticulture, UWRF Plant and Earth Science Department

"Thank you so much for being such a great company to work with. Your customer service has been wonderful. We are looking forward to developing more PACE markers linked to other disease resistant genes. Your PACE 2.0 Master Mix has worked beautifully. 3CR’s generosity to design PACE primers around SNPs is greatly appreciated. Thank you again."

Director of R&D.
Industry-leading Laboratory Service Provider

"I am very glad to see the level of support you provided on this troubleshooting, and I was happy to see such feedback from Marin who has years of experience with this assay - kudos to your team at 3CR!"

DKSH

"As a prominent player in the global life sciences industry, our longstanding collaboration with 3cr Bioscience has been instrumental to our success. Over the course of several years, we have consistently experienced exceptional delivery times and communication from their team. The level of service they provide not only meets but greatly facilitates our global ambitions. Their commitment to excellence has proven to be a valuable asset, contributing significantly to the efficiency and success of our operations on a global scale."

pace® genotyping TRIAL KIT

PCR Allele Competitive Extension

PACE® (PCR Allele Competitive Extension) Genotyping Master Mix is a patented master mix designed for use with allele-specific assays (e.g., PACE®, KASP™, Amplifluor®, etc…). It requires the addition of non-labelled oligos, unlike expensive dual-labelled probe chemistries.

PACE® Genotyping Master Mix is supplied at 2x concentration for convenience and is supplied with ROX normalising dye at either high level (500nM working concentration), low level (25nM working concentration), standard ROX (150nM working concentration) or can be supplied without ROX.

3CR Bioscience also provides a free assay design service for use with the Master Mix, with the intention that customers can save money by purchasing their own oligos.

PACE® Master Mix Trial Kits
3CR Bioscience PACE Genotyping Trial Kit

All-in-one kit to trial PACE® genotyping. Includes assays, master mix, control DNA, and a detailed setup guide.

Learn More
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END-TO-END SOLUTIONS FROM 3CR BIO

We specialize in innovative, cost-effective, end-to-end solutions for high-throughput genotyping and automation, no matter the scale.

PACE® & ProbeSure PCR Genotyping Reagents:
Ideal for ≤200 SNPs (Marker validation & Screening). 

Genotyping Lab Services:
Including DNA Extraction, Genotyping, and GBTS.

Instrumentation:
Complete high-throughput genotyping automation pipelines and individual instruments. 

Consumables:
384-well PCR plates, tubes, and seals

INTERESTED IN ANY OF THE ABOVE? GET IN TOUCH!

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Documentation
PACE User Manual
PACE Certificate

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MEET OUR TEAM

Steve AsquithManaging Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John HolmeTechnical Director

John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.

During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.

Dr. Nisha JainOperations Director

Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.

Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.

Nazma SaffinGeneral Manager
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Greig PollandAutomation and Support Manager

Greig is a hands-on automation specialist and team leader with a strong background in laboratory and industrial automation. He has spent over 25 years developing, installing, and supporting automated systems that transformed laboratory workflows. During this time, Greig worked closely with scientists and engineers to tailor automation solutions for genotyping and molecular biology, an experience that sparked his lasting passion for combining technology with practical science.

Since then, Greig has built on that foundation through leadership roles where he leads automation and support operations. He’s known for being approachable, commercially minded, and deeply committed to helping teams and customers get the best from their technology.

Whether managing a complex automation rollout or helping a customer troubleshoot in real time, Greig brings a thoughtful, collaborative approach that keeps people ,not just machines, at the centre of what he does.