Join the PACE® genotyping revolution

Transform your PCR genotyping with PACE® – the high-performance, cost-effective option. Compatible with existing marker assays, PACE genotyping reagents deliver accurate SNP and InDel marker analysis and integrate seamlessly into your workflow. Take your genotyping to the next level with our free PACE assay-design service and dedicated expert support. Join the PACE revolution today for exceptional performance, service, and value.



PACE® genotyping reagents:

  • Low Cost – half the cost of competitor mixes
  • High Performance – over a wide range of reaction volumes and throughput
  • Platform agnostic – accurate performance across different genotyping platforms
  • Expert Support –  Dedicated team with 20+ years genotyping expertise
  • Quality Assured – Every reagent batch subjected to rigorous QC procedures.


Quality, Support and Affordability are at the core of everything we do here at 3CR Bioscience. Try us and see the difference with our industry leading PCR genotyping reagents and prices now!



Transitioning to PACE is seamless. Simply use your currant PCR SNP and InDel marker assays with PACE Genotyping Master Mix on your existing thermal cycling instrument. Click here to view our comprehensive PACE Genotyping Master Mix User Guide and start saving time and money.



PACE Genotyping Master Mix
A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance.

PACE 2.0 Genotyping Master Mix
An enhanced PCR master mix for allele-specific assays. Improved signal to noise ratio and tight clustering. Developed specifically for genotyping direct from crude DNA samples.

PACE Multiplex Master Mix
For the simultaneous detection of up to four targets in a single reaction. Save time, cost, and consumables while maximising data generation.

PACE OneStep RT-PCR Master Mix
Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.

PACE Nano Genotyping Master Mix
Advanced performance at ultra-low reaction volumes. Consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.

PACE Genotyping Assays
Custom designed PACE Genotyping Assays, designed to your target sequence. Compatible with all PACE genotyping master mixes.

Patented chemistry
SNP and Indel Detection
High Performance on Crude DNA Samples
Multiplex Genotyping
Real-Time Detection
One-step RNA Genotyping
Free & Confidential Assay Design Service
Assay Sequences Provided
Cost $ $$


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David C. Zlesak, Ph.D.
Professor of Horticulture, UWRF Plant and Earth Science Department

"Thank you so much for being such a great company to work with. Your customer service has been wonderful. We are looking forward to developing more PACE markers linked to other disease resistant genes. Your PACE 2.0 Master Mix has worked beautifully. 3CR’s generosity to design PACE primers around SNPs is greatly appreciated. Thank you again."

Director of R&D.
Industry-leading Laboratory Service Provider

"I am very glad to see the level of support you provided on this troubleshooting, and I was happy to see such feedback from Marin who has years of experience with this assay - kudos to your team at 3CR!"


"As a prominent player in the global life sciences industry, our longstanding collaboration with 3cr Bioscience has been instrumental to our success. Over the course of several years, we have consistently experienced exceptional delivery times and communication from their team. The level of service they provide not only meets but greatly facilitates our global ambitions. Their commitment to excellence has proven to be a valuable asset, contributing significantly to the efficiency and success of our operations on a global scale."
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pace® genotyping TRIAL KIT

PCR Allele Competitive Extension

PACE® (PCR Allele Competitive Extension) Genotyping Master Mix is a patented master mix designed for use with allele-specific assays (e.g., PACE®, KASP™, Amplifluor®, etc…). It requires the addition of non-labelled oligos, unlike expensive dual-labelled probe chemistries.

PACE® Genotyping Master Mix is supplied at 2x concentration for convenience and is supplied with ROX normalising dye at either high level (500nM working concentration), low level (25nM working concentration), standard ROX (150nM working concentration) or can be supplied without ROX.

3CR Bioscience also provides a free assay design service for use with the Master Mix, with the intention that customers can save money by purchasing their own oligos.


We specialize in innovative, cost-effective, end-to-end solutions for high-throughput genotyping and automation, no matter the scale.

PACE® & ProbeSure PCR Genotyping Reagents:
Ideal for ≤200 SNPs (Marker validation & Screening). 

Genotyping Lab Services:
Including DNA Extraction, Genotyping, and GBTS.

Complete high-throughput genotyping automation pipelines and individual instruments. 

384-well PCR plates, tubes, and seals


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Contact us


Steve Asquith Managing Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John Holme Technical Director
John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base. During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Dr. Nisha JainOperations Director
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP. Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nazma Saffin General Manager
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Jon Curtis Non-Executive Chair
After 8 years in The Royal Air Force, Jon moved to the Imperial Cancer Research Fund where he pioneered the use of ultra high-throughput genomic automation, capable of 46,000 PCRs per hour. In the 1990’s Jon joined GlaxoSmithKline, implementing a high-throughput genomics platform into their drug discovery pipeline. Whilst there he also developed acoustic mixing into compound management, becoming the gold standard across pharma. Jon developed the world’s first commercially viable 1536-well PCR plates, automated thermal & laser plate-sealer, plus automated liquid-handling & tip washing tools to reduce waste and costs. In 2002 Jon co-founded KBioscience with Phil Robinson, utilising ultra high-throughput PCR instrumentation & a suite of automation tools to create the company’s SNPline robotic platform, with a capacity of 250,000 PCRs/day. The business was underpinned by their ground-breaking patented genotyping chemistry, KASP™, which has over 10,000 scientific papers to date. In November 2022 Jon joined 3CR Bioscience acting as an advisor bringing his commercial and scientific experience to the company.