Hydrolysis probe PCR mix for TaqMan™, BHQ®, BHQplus®, and ZEN™ assays. Offers accurate, robust performance at competitive pricing.
ProbeSure™ Master Mix delivers robust, reproducible results for SNP and Indel detection using 5’ nuclease assays. Compatible with all major probe types and qPCR systems — ready to power your workflow.
Hydrolysis probe PCR mix for TaqMan™, BHQ®, BHQplus®, and ZEN™ assays. Offers accurate, robust performance at competitive pricing.
More than 600+ Universities and Businesses trust in us.
ProbeSure™ delivers robust, probe-based PCR results with high call rates, broad compatibility, and unbeatable value — all without compromise.
ProbeSure™ works seamlessly with TaqMan™, ZEN™, BHQ®, BHQplus®, and other dual-labeled probes — no re-optimization needed.
ProbeSure master mixes are precisely optimized to perform reliably across a wide dynamic range of reaction volumes and sample template qualities.
Get premium assay quality without inflated pricing. ProbeSure™ is optimized to reduce cost per reaction while maintaining data quality.
ProbeSure™ Master Mixes have been validated across millions of reactions and trusted by hundreds of institutions worldwide.
Get the performance you expect — without the limitations. ProbeSure™ combines compatibility, multiplexing, and transparency in assay design with competitive pricing.
ProbeSure™ Master Mixes are built for precision — fully optimized for 5’ nuclease assays using TaqMan™, ZEN™, BHQ®, and BHQplus® probes. Supplied at a convenient 2x concentration, each mix comes with your choice of ROX normalization dye: high (500 nM), low (25 nM), or ROX-free — ensuring seamless integration with your qPCR setup.
Designed for performance across a wide range of reaction volumes and DNA qualities, ProbeSure™ delivers consistent results — even in demanding conditions. Whether you’re running standard qPCR, multiplex targets, or RNA-based workflows, ProbeSure™ gives you the compatibility, clarity, and cost-efficiency trusted by hundreds of labs worldwide.
| PROBESURE | COMPETITOR | |
|---|---|---|
| SNP and Indel Detection | ✓ | ✓ |
| Real-time detection | ✓ | ✓ |
| Multiplex Genotyping | ✓ | ✘ |
| One Step RNA genotyping | ✓ | ✘ |
| Cost | $ | $$ |
Legal Information
TaqMan™ is a registered trademark of Roche Molecular Systems, Inc.
Zen™ Probe is a trademark of Integrated DNA Technologies, Inc.
KASP™, BHQ™ Probes, and BHQplus™ Probes are trademarks of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
From single-target qPCR to multiplex and one-step RNA detection — explore the ProbeSure™ lineup.
Hydrolysis probe PCR mix for TaqMan™, BHQ®, BHQplus®, and ZEN™ assays. Offers accurate, robust performance at competitive pricing.
Simultaneously detect up to four targets per reaction. Streamline workflows, reduce reagent use, and maximise assay data generation.
Perform RNA reverse transcription and PCR in one step. Fast, efficient, and precise RT-PCR for probe-based genotyping.
Get the power of precise, probe-based PCR — optimized for your workflow, trusted by labs globally, and ready to ship. Choose the ProbeSure™ Master Mix that fits your assay and start generating better data today.
ProbeSure Master Mix is compatibile with most qPCR machines, thermal cyclers and fluorescence plate readers. You can check compatibility with our PACE Instrument Compatibility Guide here.
ProbeSure Multiplex Master Mix is an enhanced version of ProbeSure Master Mix, formulated to enable users to analyse up to four targets in one reaction well. For example, two bi-allelic SNPs or one reference gene and a further three genes of interest.
Users will require a plate reader capable of reading FAM, HEX, ATTO 550, ATTO 647N and ATTO 633 (the wavelengths of each of these can be found in our ProbeSure Multiplex Master Mix User Guide). ProbeSure Multiplex Master Mix is supplied at 2x concentration for convenience and is supplied with the ATTO 633 normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ATTO 633.
We are happy to send you a free sample of any of our ProbeSure master mixes to try in your lab. Please contact us at support@34bio.com to request a free trial.
Any dual-labelled probe with a fluorescent label at the 5′ end and a quencher at the 3′ end, such as TaqMan™, ZEN™ probes and BHQ® and BHQplus® probes, can all be used with ProbeSure Genotyping Master Mix.
Sign up now to receive insights, updates and industry case studies.
John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
You are currently viewing a placeholder content from Facebook. To access the actual content, click the button below. Please note that doing so will share data with third-party providers.
More InformationYou are currently viewing a placeholder content from X. To access the actual content, click the button below. Please note that doing so will share data with third-party providers.
More Information
