Universal PCR mix for allele-specific assays. Delivers precise fluorescence, high performance, and consistent genotyping across reaction volumes.
Tired of PCR reagents that deliver inconsistent results or low signal strength?
PACE MasterMix is built for labs that demand speed, accuracy, and high-throughput performance – engineered to meet the demands of fluorescence-based, high-throughput SNP genotyping.Â
Discover how PACE PCR master mixes can enhance your genotyping by cutting time and cost per reaction — without compromising quality.
Universal PCR mix for allele-specific assays. Delivers precise fluorescence, high performance, and consistent genotyping across reaction volumes.
Next-generation allele-specific PCR mix with enhanced signal clarity and robust performance, even from crude DNA samples.
Detect up to four targets in one PCR reaction. Save time, reduce consumables, and maximise throughput with effortless multiplexing.
Genotype directly from RNA. Combines reverse transcription and PCR amplification in a single, streamlined reaction.
High-performance, ultra-low-volume PCR mix for cost-effective, high-throughput genotyping in automated or miniaturised workflows.
Custom PACE® assays designed for your target sequence. Ensure reliable, accurate allele detection with full PACE® compatibility.
Fast, affordable PACE®-based screening to detect CRISPR and gene edits with high accuracy in high-throughput applications.
All-in-one kit to trial PACE® genotyping. Includes assays, master mix, control DNA, and a detailed setup guide.
Transitioning to PACE is seamless. Simply use your currant PCR SNP and InDel marker assays with PACE Genotyping Master Mix on your existing thermal cycling instrument. Click here to view our comprehensive PACE Genotyping Master Mix User Guide and start saving time and money.
Our patented PACE chemistry represents the latest advancement in allele-specific PCR, delivering the consistent, high-quality performance required for high-throughput genotyping. Each PACE PCR master mix is designed for use with PACE Genotyping Assays and is fully compatible with existing KASP™ and Amplifluor® assay workflows, making it simple to upgrade without changing your processes.
Unlike probe-based products such as TaqManâ„¢, PACE assays only require un-labelled primer oligos to make up the PACE genotyping reaction, in combination with PACE Genotyping Master Mix. Watch our quick video explaining how PACE genotyping works.
All PACE PCR master mixes are supplied at a convenient 2× concentration and include ROX normalising dye options to match your instrument requirements – high (500 nM), low (25 nM), standard (150 nM), or no ROX.
Boost Your Genotyping Workflows with PACE® Multiplex!
Looking to double your data output while cutting down on DNA, consumables, and labour?
PACE® Multiplex Master Mix makes it effortless—no optimisation required!
| PACE | COMPETITOR | |
|---|---|---|
| Patented chemistry | ✓ | ✓ |
| SNP and Indel Detection | ✓ | ✓ |
| High Performance on Crude DNA Samples | ✓ | ✘ |
| Multiplex Genotyping | ✓ | ✘ |
| Real-Time Detection | ✓ | ✘ |
| One-step RNA Genotyping | ✓ | ✘ |
| Free & Confidential Assay Design Service | ✓ | ✘ |
| Assay Sequences Provided | ✓ | ✘ |
| Cost | $ | $$ |
Legal Information
KASPâ„¢ is a trademark of LGC Biosearch Technologies
Amplifluor® is a registered trademark of Merck KGaA
TaqManâ„¢ is trademark of Roche Molecular Systems, Inc.
A PCR master mix brings together the essential ingredients needed for robust, consistent amplification, removing the variability that comes with preparing each component separately. The core components are always the same, but the quality and balance of those components determine how well your reactions perform.
In a typical PCR master mix, you’ll find a thermostable DNA polymerase, dNTPs, magnesium ions, and a carefully optimised buffer system to support consistent enzyme activity. Many mixes, including the PACE range, also include stabilisers to maintain performance across different reaction volumes, plus optional ROX dye levels to suit a variety of instruments. How these components are formulated makes a significant difference, especially in fluorescence-based SNP genotyping, where signal clarity and clustering matter.
PACE PCR master mixes are built around this principle: the right components, balanced precisely, to deliver clean data and reliable results for high-throughput workflows.
We specialize in innovative, cost-effective, end-to-end solutions for high-throughput genotyping and automation, no matter the scale.
PACE® & ProbeSure PCR Genotyping Reagents:
Ideal for ≤200 SNPs (Marker validation & Screening).Â
Genotyping Lab Services:
Including DNA Extraction, Genotyping, and GBTS.
Instrumentation:
Complete high-throughput genotyping automation pipelines and individual instruments.Â
Consumables:
384-well PCR plates, tubes, and seals
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John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
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