News & Events


Exciting news! 3CR Bioscience proudly unveils its latest offering – our range of PACE PCR Genotyping Trial Kits. These kits of designed for anyone who wants to try PACE genotyping reagents in their lab for the first time, offering a set of validated DNA samples, SNP assays, and PACE Master Mix. This comprehensive kit is meticulously crafted to provide researchers with all the necessary tools for a seamless trial experience, allowing you to experience a new era of genotyping precision.


The PACE allele-specific PCR mechanism is a robust methodology utilised for the detection of Single Nucleotide Polymorphisms (SNPs), Insertions and Deletions (Indels), and other sequence variants. For detailed insights into the PACE genotyping chemistry visit our PACE product homepage or read our tech note on SNP and Indel genotyping with PACE.


The PACE Genotyping Trial Kits contain everything you need to run a trial PACE® allele-specific PCR Genotyping Reaction on your existing lab equipment.  Available for PACE Genotyping Master Mix, PACE 2.0 Genotyping Master Mix, PACE Multiplex Master Mix, and PACE Nano Genotyping Master Mix.

Inside the kit, you’ll find:

  1. Test DNA Samples: Three carefully validated DNA samples (DNA 1, 2, and 3), each representing distinct genotypes, conveniently packaged for immediate use.
  2. PACE Genotyping Assays: A precisely formulated PACE Genotyping Assay, packaged in a single tube with a 2D barcode for easy identification.
  3. PACE Master Mix: The PACE Genotyping Master Mix or its variants – PACE 2.0 Genotyping Master Mix, PACE Multiplex Master Mix, or PACE Nano Genotyping Master Mix, ensuring flexibility and compatibility with diverse experimental requirements.
  4. Comprehensive Manual: A detailed PACE Genotyping Trial Kit Manual, providing step-by-step instructions for seamless experimentation.


This trial kit is tailored for researchers seeking to explore the capabilities of PACE genotyping reagents in their laboratories. Whether you’re a seasoned scientist looking to adopt a new genotyping method or a novice researcher venturing into the realm of genomics, the PACE PCR Genotyping Trial Kit offers an ideal starting point.


Running a trial PACE Genotyping Reaction using the kit is a straightforward process:

  1. Dispense DNA Samples: Dispense the provided DNA samples (DNA 1, 2, and 3) along with a no-template control (water) in triplicate onto a PCR plate, following suggested volumes.
  2. Prepare PACE Reaction Mix: Combine the appropriate volumes of PACE Genotyping Master Mix with the PACE Genotyping Assay as directed, ensuring thorough mixing.
  3. Dispense PACE Reaction Mix: Dispense the prepared mixtures into each well containing DNA samples, adhering to indicated volumes, to complete the PACE Genotyping Reaction setup.
  4. Seal and Centrifuge: Seal the PCR plate with an optically clear seal and centrifuge to ensure proper mixing and distribution of components.
  5. Thermal Cycling: Execute thermal cycling of the reaction plate using provided cycling conditions.
  6. Data Analysis: Read the plate and compare the generated data with expected results outlined in the manual, simplifying result interpretation.


To facilitate successful experimentation with the PACE PCR Genotyping Trial Kit, ensure the availability of the following components:

  • qPCR machine or Thermocycler plus a fluorescent plate reader.
  • PCR plate or equivalent, along with an appropriate optically clear seal.
  • PCR-grade water for dilutions and reaction setup.


With the introduction of the PACE PCR Genotyping Trial Kit, 3CR Bioscience continues its legacy of innovation and commitment to advancing genotyping technologies. Experience the next generation of PCR genotyping with confidence and precision. Embrace the future of genomics with the PACE PCR Genotyping Trial Kit – your gateway to unparalleled genotyping excellence.

Order a free PACE Genotyping Trial Kit now

PACE PCR Genotyping Trial Kits
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Contact us


Steve Asquith Managing Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John Holme Technical Director
John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base. During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Dr. Nisha JainOperations Director
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP. Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nazma Saffin General Manager
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Jon Curtis Non-Executive Chair
After 8 years in The Royal Air Force, Jon moved to the Imperial Cancer Research Fund where he pioneered the use of ultra high-throughput genomic automation, capable of 46,000 PCRs per hour. In the 1990’s Jon joined GlaxoSmithKline, implementing a high-throughput genomics platform into their drug discovery pipeline. Whilst there he also developed acoustic mixing into compound management, becoming the gold standard across pharma. Jon developed the world’s first commercially viable 1536-well PCR plates, automated thermal & laser plate-sealer, plus automated liquid-handling & tip washing tools to reduce waste and costs. In 2002 Jon co-founded KBioscience with Phil Robinson, utilising ultra high-throughput PCR instrumentation & a suite of automation tools to create the company’s SNPline robotic platform, with a capacity of 250,000 PCRs/day. The business was underpinned by their ground-breaking patented genotyping chemistry, KASP™, which has over 10,000 scientific papers to date. In November 2022 Jon joined 3CR Bioscience acting as an advisor bringing his commercial and scientific experience to the company.