Affordable, High-Performance Genotyping for Vegetable Crops

Unlock Better Varieties with PACE® by 3CR

Vegetable breeding plays a crucial role in global food and nutrition security. With increasing demand for resilient, high-quality crops that meet both consumer expectations and producer needs, breeders require genotyping solutions that are accurate, scalable, and cost-effective.

At 3CR, we help breeders and researchers overcome these challenges. Our patented PACE® (PCR Allelic Competitive Extension) chemistry provides reliable, high-throughput SNP genotyping that supports marker-assisted selection, trait mapping, and variety verification in vegetables. By making SNP genotyping more affordable, PACE® enables faster delivery of improved varieties to the market.

MARKER-ASSISTED SELECTION

Applications in Vegetable Genotyping

Vegetable breeders and researchers use PACE® across a wide range of critical applications:

Marker-Assisted Selection

Identify plants carrying favourable alleles for traits such as nutrient content, uniformity, or stress resistance.

Applications in Vegetable Genotyping

Vegetable breeders and researchers use PACE® across a wide range of critical applications:

Trait Mapping & QTL Analysis

Link SNP markers to key agronomic traits such as root shape, leaf colour, or flowering time, enabling more efficient selection.

Applications in Vegetable Genotyping

Vegetable breeders and researchers use PACE® across a wide range of critical applications:

Genomic Selection

Incorporate SNP data into prediction models to accelerate breeding cycles and deliver improved varieties sooner.

Applications in Vegetable Genotyping

Vegetable breeders and researchers use PACE® across a wide range of critical applications:

Disease Resistance Screening

Identify resistance-associated SNPs against pathogens such as downy mildew, Fusarium, or bacterial wilt to strengthen crop resilience.

Applications in Vegetable Genotyping

Vegetable breeders and researchers use PACE® across a wide range of critical applications:

Variety Verification & Seed Purity Testing

Confirm hybrid seed purity, validate parentage, and ensure consistency across breeding and production pipelines.

Applications in Vegetable Genotyping

Vegetable breeders and researchers use PACE® across a wide range of critical applications:

Germplasm Characterisation

Assess genetic diversity in breeding collections, landraces, or gene bank accessions to preserve and utilise valuable genetic resources.

Vegetable-Specific Examples

  • Tomato & Pepper: SNP assays for fruit quality, yield, and disease resistance (e.g., Tomato Yellow Leaf Curl Virus).
  • Cucumber & Melon: Genotyping for fruit shape, flavour, and stress tolerance.
  • Brassicas (cabbage, broccoli, cauliflower): Selection for disease resistance and nutritional traits.
  • Carrot & Beet: Marker-assisted breeding for root colour, shape, and sugar content.
  • Leguminous Vegetables (bean, pea): Screening for pest resistance, drought tolerance, and protein content.

Already Sure? Let’s UPGRADE Vegetable Genotyping TOGETHER!

PACE® provides flexibility across the entire vegetable sector, supporting species-specific breeding challenges and broad genetic improvement goals.

Order your PACE® Master Mix now and streamline your workflows with unmatched speed, accuracy, and cost-efficiency—trusted by 600+ labs worldwide.

Streamlined for Breeding Pipelines

PACE® integrates easily into existing PCR workflows and is compatible with most qPCR instruments. Our mixes are supplied at 2x concentration, with or without ROX dye, and require no complex optimisation.

For breeding groups without in-house PCR capacity, 3CR offers dependable outsourced genotyping with PACE® chemistry to support your breeding programmes.

Get Started with PACE® for Vegetables

Whether you’re developing climate-resilient varieties, extending shelf life, or enhancing consumer appeal, 3CR offers affordable genotyping solutions to help achieve your breeding objectives.

MID- & HIGH-DENSITY GBTS MARKER PANELS FOR VEGETABLE GENOTYPING

Our targeted SNP marker panels deliver precise genotyping for selective breeding, varietal identification, and trait improvement in vegetables. Cost-effective, species-specific, and designed to support high-throughput research and commercial breeding programs.

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End-to-end genotyping solutions FOR AQUACULTURE BREEDING

Unlock the full spectrum of genotyping possibilities with our comprehensive, modular range of instruments. Explore our end-to-end genotyping automation solutions and discover the perfect fit for your laboratory, ensuring efficiency and accuracy at every step.

AUTOMATED EXTRACTION

Automated Extraction

Fully automated, high-quality nucleic acid extraction & purification instrumentation for use with magnetic bead-based reagents. Suitable for genotyping, library sequencing, gene chip analysis & more.

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LIQUID HANDLING

LIQUID HANDLING

Automated liquid handling workstation for sample replication and reformatting, low volume assay dispensing, plate sealing and more. On-deck sonicating washing dramatically reduces tip consumption and cost.

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THERMAL CYCLING

THERMAL CYCLING

A highly efficient, high-throughput thermal cycling water bath instrument capable of running up to 25 PCR plates per run. Robust, streamlined basket design.

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DATA ANALYSIS

DATA ANALYSIS

A high-speed plate scanner for precise fluorescence signal detection and analysis. Full-field scanning technology for excellent sensitivity and resolution; automated loading, detection and unloading.

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About 3CR

3CR is committed to Making Science Affordable. Based in the UK, we provide our patented PACE® technology to breeders and researchers across agriculture, aquaculture, and life sciences. By delivering high-performance, cost-effective SNP genotyping chemistry, we enable our customers to accelerate genetic progress across vegetables, fruits, crops, and livestock.
PACE genotyping accuracy, independently verified
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YEARS OF COMBINED TECHNICAL EXPERIENCE
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customers worldwide
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assays designed to date
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seed producers worldwide trust PACE genotyping
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Take the Next Step! Vegetable breeding demands precision and efficiency.

With PACE® from 3CR, you can lower costs, increase throughput, and deliver better varieties to growers and consumers worldwide.

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MEET OUR TEAM

Steve Asquith Managing Director
Steve began his career in the Genetics Division of GlaxoSmithKline, as part of the team establishing GSK’s high-throughput core genotyping laboratory. Steve joined KBioscience when it was first founded in 2002 and was a key driver in taking the company from a small start-up to a multi-national service laboratory, quickly growing the company’s revenue to over $7.5M p.a. Following the acquisition of Kbioscience by LGC in 2011, Steve was appointed Global Director of Operations for LGC Genomics, responsible for over 100 staff in Europe and N. America, successfully elevating the genotyping products and service business. Steve held a crucial leadership role until he left in 2016. In 2017 Steve joined forces with John Holme to create 3CR Bioscience, a new company with a mission to deliver outstanding, customer-focused genotyping products with innovation and affordability at its core.
Dr. John Holme Technical Director
John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base. During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASPâ„¢, KlearKall, KlearGene, KlearAmp and KlearTaqâ„¢, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Dr. Nisha JainOperations Director
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP. Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nazma Saffin General Manager
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Jon Curtis Non-Executive Chair
After 8 years in The Royal Air Force, Jon moved to the Imperial Cancer Research Fund where he pioneered the use of ultra high-throughput genomic automation, capable of 46,000 PCRs per hour. In the 1990’s Jon joined GlaxoSmithKline, implementing a high-throughput genomics platform into their drug discovery pipeline. Whilst there he also developed acoustic mixing into compound management, becoming the gold standard across pharma. Jon developed the world’s first commercially viable 1536-well PCR plates, automated thermal & laser plate-sealer, plus automated liquid-handling & tip washing tools to reduce waste and costs. In 2002 Jon co-founded KBioscience with Phil Robinson, utilising ultra high-throughput PCR instrumentation & a suite of automation tools to create the company’s SNPline robotic platform, with a capacity of 250,000 PCRs/day. The business was underpinned by their ground-breaking patented genotyping chemistry, KASP™, which has over 10,000 scientific papers to date. In November 2022 Jon joined 3CR Bioscience acting as an advisor bringing his commercial and scientific experience to the company.