Free assay design

Free assay design service

Save money to spend on valuable research by designing assays with us and purchasing oligos yourself, rather than paying a premium for someone else to do this for you.

Existing Master Mix customers can either fill in the design form below, or email 3CR Bioscience with your sequence and targeted polymorphism, and we’ll design oligos that are compatible with chemistries such as PACE™, KASP™, Amplifluor®, etc… 

Why use our free assay design service?

There are two simple reasons:

  • Cost – it is cheaper to order oligos individually from an oligo company such as IDT than it is to order ready made assays.
  • Experience – our design team has in excess of 40 years experience of designing and optimising primers for use in allele-specific PCR

How to submit your sequences

Sequences can be submitted using the below form, or emailed either in Excel or as a text file.

If required, a NDA can be signed in advance of you submitting sequences. Please contact us to arrange this.

(* required field)

Please input your sequence name on one line, the actual sequence on the next and an empty line before the next sequence name, etc... Targets should should be identified by squared brackets (e.g. ACT[G/T]TGC)

  • Excel
    • Column A – this should contain your sequence name
    • Column B – this should contain your sequence
    • SNP identifier – identify the two bases of the SNP with squared brackets and a forward slash e.g.[A/G] or squared brackets containing the IUPAC code of the SNP e.g. [R]
    • Other surrounding polymorphisms should be identified by their IUPAC code (see table below) without squared brackets
    • Each new SNP target should be submitted on a new row
    • Example Excel submission
  • Text
    • First line – this should contain your sequence name
    • Second line – this should contain your sequence
    • SNP identifier – identify the two bases of the SNP with squared brackets and a forward slash e.g.[A/G] or squared brackets containing the IUPAC code of the SNP e.g. [R]
    • Other surrounding polymorphisms should be identified by their IUPAC code (see table below) without squared brackets
    • Leave a blank line between each target sequence.

IUPAC codes

IUPAC nucleotide code Base
A Adenine
C Cytosine
G Guanine
T (or U) Thymine (or Uracil)
R A or G
Y C or T
S G or C
W A or T
K G or T
M A or C
B C or G or T
D A or G or T
H A or C or T
V A or C or G
N any base

 

With our relationship with Integrated DNA Technologies (IDT) now well established, we continue to provide free PACE™ assay designs. We now also provide a pre-populated IDT ordering form for you to order your oligos from IDT and to receive the assays ready mixed in a plate, should you choose.