Information

PACE™ Genotyping Master Mix

Product information

PACE™ (PCR Allele Competitive Extension) Genotyping Master Mix is a universal master mix designed for use with allele-specific assays (e.g. KASP™, Amplifluor®, etc…).  3CR Bioscience also provides a free assay design service for use with the Master Mix, with the intention that customers can save money by purchasing their own oligos.  PACE™ Genotyping Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500nM working concentration), low level (25nM working concentration), with standard ROX (150nM working concentration) or without ROX.

Why use PACE™ Genotyping Master Mix?

The benefits of PACE™ Genotyping Master Mix over other mixes (such as KASP™, Amplifluor®, etc…) are improved reduction in non-specific amplification, only one master mix, independent of  reaction volumes, and very significantly lower cost.

Ordering

PACE-IR Genotyping Master Mix

Product information

PACE-IR™ (PCR Allele Competitive Extension) Genotyping Master Mix is a version of PACE™ Genotyping Master Mix, formulated to neutralise the effect of PCR inhibitors in crude DNA extractions.

PACE-IR™ Genotyping Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500nM working concentration), low level (25nM working concentration), standard ROX (150nM working concentration) or without ROX.

Why use PACE-IR™ Genotyping Master Mix?

The standard PACE™ product will function well with purified DNA samples, as well as most crude DNA extractions, whereas PACE-IR™ is designed specifically for use where high residual levels of inhibitors in the DNA samples are adversely affecting the PCR.

Ordering

PACE™ assays

Product information

Why use PACE™ assays?
PACE™ assays work in the same way as KASP™ assays (same tail sequences), and existing KASP™ assays are compatible with PACE™ Genotyping Master Mix. Rather than charge customers for the supply of the assay, we provide the oligo sequences for customers to purchase themselves.

Free assay design service

ProbeSure™ Genotyping Master Mix

Product information

ProbeSure™ Genotyping Master Mix is designed for use with 5’ nuclease assays and is suitable for use with TaqMan™, BHQ®, BHQplus®, Zen™ probes. ProbeSure™ Genotyping Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500nM final concentration), low level (25nM final concentration) or without ROX.

Why use ProbeSure™ Genotyping Master Mix?
ProbeSure™ Genotyping Master Mix has been carefully optimised to work over a wide dynamic range of reaction volumes and sample template qualities.  ProbeSure™ Genotyping Master Mix combines class-leading performance with the most competitive prices in the market.

Ordering

ProbeSure-IR™ Genotyping Master Mix

Product information

ProbeSure-IR™ Genotyping Master Mix is a version of ProbeSure™ Genotyping Master Mix, formulated to neutralise the effect of PCR inhibitors in crude DNA extractions.

ProbeSure-IR™ Genotyping Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500nM working concentration), low level (25nM working concentration) or without ROX.

Why use ProbeSure-IR™ Genotyping Master Mix?
The standard ProbeSure™ product will function well with purified DNA samples, as well as most crude DNA extractions, whereas ProbeSure-IR™ is designed specifically for use where high residual levels of inhibitors in the DNA samples are adversely affecting the PCR.

Ordering