
Convert GWAS Discoveries into Robust Molecular Markers with PACE®
Discover how PACE® genotyping helps breeders turn GWAS discoveries into fast, scalable, and cost-effective molecular screening tools for real-world breeding success.

About the author
Dr Sarah Holme
Scientist, 3CR Bioscience
Dr Sarah Holme is a scientist at 3CR Bioscience, where she develops and communicates the company's PCR genotyping technology and its applications in plant and animal breeding. She brings over 20 years of biotechnology experience, from research on novel antibacterial therapies to new product development for genotyping and real-time PCR detection chemistries. Alongside her lab career, she has worked extensively as a science writer and editor, producing application notes, case studies and technical content for the life sciences industry.

Discover how PACE® genotyping helps breeders turn GWAS discoveries into fast, scalable, and cost-effective molecular screening tools for real-world breeding success.

Discover seven common plant and seed DNA extraction challenges—and how optimised chemistry combined with MagC 384 automation helps deliver consistent, high-quality DNA at scale.

Discover how PACE® genotyping helped researchers validate parent-of-origin effects in pigs and why targeted genotyping remains essential for turning genomic discoveries into actionable insights.

What if you could run thousands of PCR reactions using a fraction of the reagents—miniaturisation is transforming genotyping at scale.

Learn how PACE genotyping is helping avocado breeders accelerate marker-assisted selection for commercially valuable skin colour traits.

Unlock faster, smarter breeding decisions with early sex determination powered by scalable PACE genotyping.

What if breeders could identify disease-resistant watermelon plants before they even grow – this study shows how PACE® genotyping makes it possible.

Learn how to design highly accurate SNP and indel genotyping assays using proven PACE strategies.

Learn how Biomark X9 and PACE technology enable scalable, high-throughput SNP genotyping for modern genomics and breeding workflows.

Should you build an in-house SNP genotyping lab or outsource it? Discover when outsourcing accelerates research, reduces costs, and improves scalability.

Plant DNA extraction can be challenging — learn how to optimise DNA extraction kits for difficult tissues, seeds, and inhibitor-rich plant species.

Discover how PACE® genotyping transforms CRISPR edit screening into a fast, scalable, and cost-effective workflow for agriculture and life sciences.

Generate twice the SNP genotyping data in a single reaction while cutting DNA usage and costs in half.

A practical walkthrough of PCR steps in PACE® genotyping, from sample prep to fluorescence readout.

Learn how PACE® genotyping enables faster, more accurate hemp breeding.

Learn how PACE® genotyping bridges the gap between GWAS discovery and marker-assisted selection in genetically complex, polyploid rose breeding.

Discover when manual pipetting is enough—and why automated pipetting with 3CR’s GeneArrayer and PACE® genotyping delivers superior speed, accuracy, and scalability.

Discover how high-throughput PCR using PACE® genotyping accelerates CRISPR screening and genome editing verification across large sample populations.

Learn how Cornell University uses PACE® SNP genotyping to accelerate hemp breeding—enabling early trait selection, sex determination, and cost-effective genetic screening.

Discover how PACE® genotyping revealed a new sweetness QTL in white lupin, supporting ultra-low-alkaloid breeding and sustainable protein production.

Improving poultry immunity starts with understanding genetic diversity. Explore how researchers are using modern SNP tools to reveal what’s been hidden in the genome.

Discover how new HNMT gene markers can boost carnosine levels and naturally enhance poultry meat flavor.

Researchers have developed a rapid, PACE-validated sex-marker panel for Tambaqui, enabling fast and accurate gender identification to boost efficiency and genetic gains in aquaculture breeding programs.

Researchers have developed a rapid, PACE-validated sex-marker panel for Tambaqui, enabling fast and accurate gender identification to boost efficiency and genetic gains in aquaculture breeding programs.

Discover how PACE genotyping streamlines marker-assisted selection and accelerates the development of high-performing cereal varieties.

PACE OneStep RT-PCR delivers fast, low-cost SNP genotyping direct from RNA to track emerging SARS-CoV-2 variants in real time.

Compare PACE® and PACE® 2.0 to see which master mix delivers the performance, sensitivity, and value your genotyping workflow needs.

A new PACE genotyping assay delivers an accurate, affordable, and scalable method to identify male and female hop plants early in breeding programs — enabling faster selection, greater efficiency, and a 97% female yield in field trials.

Using a reverse genetic screen, researchers at INRAE have uncovered recessive lethal variants linked to lamb mortality in French dairy sheep breeds. Discover how advanced genomic tools and PACE® genotyping are helping improve animal health, breeding outcomes, and herd sustainability.

Genotyping is a vital tool in modern biology, helping researchers analyze DNA with speed and precision. From detecting disease mutations to improving crops, the applications of genotyping are wide-ranging. But what is genotyping used for, and how do methods like SNP assays deliver value?

Genotyping and sequencing are both powerful genomic tools — but they serve different research goals. Learn how PCR-based genotyping compares to next-generation sequencing (NGS) in cost, speed, data depth, and scalability, and discover when each method delivers the best results.

SNP genotyping is transforming genetics across agriculture, aquaculture, and life sciences. Learn how smart assay design, allele-specific PCR, and 3CR’s PACE® technology deliver high-throughput, precise, and cost-effective genotyping for any research or breeding program.

Rapid PCR-based pathogen detection is revolutionising diagnostics in healthcare, agriculture, and environmental monitoring. Discover how 3CR’s PACE® and PACE OneStep RT-PCR systems deliver fast, accurate, and scalable detection of bacteria, viruses, and other pathogens — helping safeguard global health and biosecurity.

A general introduction to PCR master mixes followed by information on 3CR Bioscience’s PACE PCR Master Mixes, which provide cost-effective solutions for accurate SNP and Indel genotyping, using patented fluorescent technology for high-throughput, reliable allele-specific PCR analysis.

PCR instrumentation has evolved from manual setups to automated systems, enhancing efficiency, accuracy, and scalability in genotyping. 3CR Bioscience offers advanced solutions for PCR instrumentation and integration into a wide range of workflows.

PCR genotyping is a fast, cost-effective method for validating genetic markers. Using technologies like PACE®, it enables precise, high-throughput SNP analysis for breeding and genetic research programs.

3CR Bioscience’s GeneExtract H9600 and H384 offer fast, cost-effective, high-throughput automated DNA extraction, delivering superior purity and efficiency for genotyping, sequencing, and biotech applications.

Genome editing, especially with CRISPR/Cas systems, is revolutionizing genetic research. Its applications range from enhancing crop traits in agriculture to advancing cell line development in

Our innovative genotyping instrumentation range is revolutionising high-throughput genomics across agriculture and life sciences. Paired with our patented PACE® genotyping reagents, these state-of-the-art tools are enhancing

A new study by Clare et al. has made significant strides in understanding the genetic basis of disease susceptibility in hybrid barley. A collaboration between

In genetic research, particularly within breeding programs designed to boost crop yields or enhance animal traits, the identification and validation of genetic markers are crucial.

Enhancing Animal Breeding Programs PACE genotyping (PCR Allele Competitive Extension) has become a cornerstone technology in animal genomics, offering precise, rapid, and cost-effective solutions for

Crude DNA extraction is a streamlined approach that allows researchers to prepare DNA for PCR genotyping without the time, cost, and complexity of full nucleic

Discover the latest advances in plant breeding with our blog on newly released PACE SNP markers for Cannabis sativa. Learn how these innovative tools improve

Multiplex Allele-Specific PCR Has Arrived In the realm of genetic research and molecular biology, accuracy and efficiency are paramount. Scientists are constantly seeking innovative solutions

In the fast-growing world of genomics and genotyping, having a reliable partner by your side is paramount. Enter 3CR Bioscience, your dedicated ally committed to

PACE® – What is allele-specific PCR? Allele-specific PCR distinguishes between two versions of a single DNA base by using primers that match one allele exactly.

How allele-specific PCR and SNP markers are transforming peanut cultivar development 3CR was delighted to host the Industry Workshop ‘Accelerating Crop and Animal Breeding Enhancement

Empowering Global Efforts in Sustainability and Diversity with SNP Genotyping There is a wide diversity of animal genetic resources available for genomics and breed improvement

Explore our New Tech Note! Discover the secrets of successful genotyping with our Tech Note ‘Top 5 Assay Designs for SNP Genotyping and Indel Genotyping’. This comprehensive guide takes

SNP Genotyping pushes modern breeding of hops for brewing and beyond In hop cultivation, identifying female plants is crucial for efficient production. Traditionally, determining a

Identifying Candidate Genes and Validating Markers for Anthracnose Resistance Through QTL Analysis and PACE PCR Genotyping. In a study published last month in Frontiers in Plant Science,
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John joined KBioscience shortly after it was founded, in 2003, and became Head of Technical Development, building the company’s genotyping and DNA extraction product portfolio and service delivery until 2011 when it was acquired by LGC. Post-acquisition, John was appointed Head of Technical Group for LGC Genomics, in charge of all Research & Development and Technical Support activities for the company. In this role John continued to build on the high-quality products and services provided to the companies growing customer base.
During the 19 years John has worked in commercial R&D, he has co-invented numerous highly successful products including PACE®, ProbeSure, KASP™, KlearKall, KlearGene, KlearAmp and KlearTaq™, creating breakthrough offerings in genotyping and extraction and generating huge revenues for the companies he has worked in. In 2017, he joined forces with Steve Asquith and started 3CR Bioscience. John is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
Nisha has been innovating since the start of her career at Geneform Technologies developing Iso-thermal Genotyping Technologies. Nisha joined KBioscience in 2008, as Senior R&D Scientist and key account Technical Support Scientist, developing KASP and Klearkall performance and coinventing two further versions of KASP.
Nisha has more than 15 years’ experience working in molecular biology and genotyping technologies, with extensive experience in the areas of R&D, Quality Assurance and Customer Technical Support. She has technically assisted many giants of the industry with their protocol development and troubleshooting and continues to deliver high-quality support and guidance. In 2018, Nisha joined 3CR Bioscience as Operations Director where she continues to develop PACE and ProbeSure for an increasing range of applications, and to grow 3CR Bioscience’s new product pipeline. Nisha is dedicated to developing outstanding, innovative genotyping products and providing the very best technical support to customers globally.
For 20 years Nazma Saffin has worked and gained extensive expertise within the genotyping sector. Working at Kbioscience and then LGC, she has held operational leadership posts responsible for manufacturing and laboratory services. With experience of ISO 9001 implementation, production scale up and LEAN operations, Nazma has successfully led highly profitable production departments. Joining 3CR Bioscience in 2022, Nazma is committed to delivering operational excellence.
Greig is a hands-on automation specialist and team leader with a strong background in laboratory and industrial automation. He has spent over 25 years developing, installing, and supporting automated systems that transformed laboratory workflows. During this time, Greig worked closely with scientists and engineers to tailor automation solutions for genotyping and molecular biology, an experience that sparked his lasting passion for combining technology with practical science.
Since then, Greig has built on that foundation through leadership roles where he leads automation and support operations. He’s known for being approachable, commercially minded, and deeply committed to helping teams and customers get the best from their technology.
Whether managing a complex automation rollout or helping a customer troubleshoot in real time, Greig brings a thoughtful, collaborative approach that keeps people ,not just machines, at the centre of what he does.
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